The ligation reaction was assembled according to Protocol 11. Both the plasmid samples were used to recircularization. The reaction tubes was kept O/N at 4°C.
Gabriel
finO and finP - Still Trying to Confirm our Biobricks
Today we performed minipreps from yesterday's inoculated cultures, according to Protocol 2.
Marcelo
CeaB and CeiB: colony PCR
We got apparently good transformations but we have to prove the correct position of DNA inserted. So, we performed colony PCR with right primers.
We didn’t get the band size expected and we found unspecific amplification, therefore we will make miniprep and we will try PCR again.
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