Project > OR Gate and Output
Design
The OR gate and output module is constructed as below:
Salicylate promter is placed upstream of the PO promoter, the double promoter construct can act as an OR Gate, either PhiR73 or salicylate can activate the promoter.
In [http://parts.mit.edu/igem07/index.php/Cambridge/Amplifier_project Cambridge 2007 Amplifier project], the PhiR73 activator and the PO promoter is used to amplify signal from an inducible promoter.
 fig2. Cambridge 2007 amplifier project picture from [http://parts.mit.edu/igem07/index.php/Cambridge/Amplifier_project Cambridge 2007 page] In order to make the transcription level from PO promoter similar to that of the sal promoter, the activator is designed to be on a lower copy plasmid than the reporter. This is also discussed in the second stage assembly section.
Testing
In order to test whether the OR Gate works, we constructed the following 2 strains of E.coli to test each of the two promoters.
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