Team:Kyoto/GSDD/Notebook/0810-0816

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0727-0802
Construction.
0803-0809
Construction.
0810-0816
Construction.
0817-0823
Construction.PCR.
0824-0830
Construction.PCR.
0831-0906
Construction.PCR.MPR.
0907-0913
Construction.PCR.MPR.
0914-0920
Construction.PCR.MPR.
0921-0927
Construction.PCR.MPR.
0928-1004
Construction.PCR.MPR.
1005-1011
Construction.PCR.MPR.
1012-1018
Construction.MPR.Observation.

0803-0809 : what to do. what to do. what to do. what to do.

Monday, 10 August - Friday, 14 August

To Do

  • Transformation
    • 1-N-1
    • 1-K-14
    • 2-N-13

Results

colony

Kyoto 0810.jpg

1-N-1
observed
1-K-14
observed
2-N-13(1)
none
2-N-13(2)
none
2-N-13(3)
none

We didn’t check that 2-N-13 was Kanamycin resistant at first(1,2). We made LB plate(+Kan), and tried the transformation again(3). However, no colony was observed. PCR amplification is required for amplifying 2-N-13.


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