We decided to make cDNA synthesis of the 4 samples of RNA (Blood1, Blood3, Spleen1 and Oviduct2). We used both pDN6 and oligodT primers for the synthesis.
Taís
RBS+CFP ligation
We performed a new digestion of the plasmids containing the ligation RBS+CFP. We followed Protocol 14. The digestion lasted four hours and we used less plasmid comparing to the first digestion.
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