Team:Paris/constructions

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Constructions

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Vector	Insert	PCR product	Plasmid	Strains	Kanamycin	Ampicilin	Chloranphenicol

Plasmid A

Designer	Parts description	Corresponding BioBricks	Status (last update)	Plasmid #	Registry
CC	(Pbad :: rbs - lacI - LVA) in [http://partsregistry.org/Part:pSB1A3 pSB1A3]???Pbad is on PSB2K3	[http://partsregistry.org/Part:BBa_I0500 Bba_I0500] :: [http://partsregistry.org/Part:BBa_J24679 BBa_J24679] in [http://partsregistry.org/Part:pSB2K3 pSB2K3]	pSB1A3 transformed, digested, PBad transformed and not digested well- LacI transformed and digested (10/08/09)	??	BBa_????
CC	(Pbad :: rbs - lacI - LVA) :: ter in [http://partsregistry.org/Part:pSB1A3 pSB1A3]	BBa_??? :: [http://partsregistry.org/Part:BBa_B0014 BBa_B0014] in [http://partsregistry.org/Part:pSB2K3 pSB2K3]	ter transformed, digested and purified (10/08/09)	??	BBa_????
CC	((Pbad :: rbs - lacI - LVA) :: RFP) in [http://partsregistry.org/Part:pSB1A3 pSB1A3]	BBa_??? :: [http://partsregistry.org/wiki/index.php?title=Part:BBa_K093005 BBa_K093005] in [http://partsregistry.org/Part:pSB2K3 pSB2K3]	pSB2K3 transformed and digested, RFP : primers designed but not yet ordered (10/08/09)	??	BBa_????
CC	(((Pbad :: rbs - lacI - LVA) :: RFP) :: ter) in [http://partsregistry.org/Part:pSB1A3 pSB1A3]	BBa_??? :: [http://partsregistry.org/Part:BBa_B0014 BBa_B0014] in [http://partsregistry.org/Part:pSB2K3 pSB2K3]		??	BBa_????
CC	(OmpA Linker :: g3p) in [http://partsregistry.org/Part:pSB2K3 pSB2K3]	[http://partsregistry.org/Part:BBa_K103006 BBa_K103006] :: BBa_??? in [http://partsregistry.org/Part:pSB2K3 pSB2K3]	OmpA linker standardisé - OmpA amplified, not purified - Same for g3p (10/08/09)	??	BBa_????
	(g3p :: clyA) in [http://partsregistry.org/Part:pSB2K3 pSB2K3]	BBa_??? :: BBa_??? in [http://partsregistry.org/Part:pSB2K3 pSB2K3]	autre possibilité (souf)- 1 primer missing for C term fusion with clyA (10/08/09)	??	BBa_????
CC!	((OmpA Linker :: g3p) :: ter) in [http://partsregistry.org/Part:pSB2K3 pSB2K3]	BBa_??? :: [http://partsregistry.org/Part:BBa_B0014 BBa_B0014] in [http://partsregistry.org/Part:pSB2K3 pSB2K3]		??	BBa_????
CC!	((???autotransporter??? - jun/foss) :: ter) in [http://partsregistry.org/Part:pSB2K3 pSB2K3]	BBa_??? :: [http://partsregistry.org/Part:BBa_B0014 BBa_B0014] in [http://partsregistry.org/Part:pSB2K3 pSB2K3]	jun/foss has to be synthetized & autotransporter to be found!!!! (10/08/09)	??	BBa_????
CC!	((rbs - fecA) :: ((rbs - jun/foss) :: ter)) in [http://partsregistry.org/Part:pSB2K3 pSB2K3]	BBa_??? :: BBa_??? in [http://partsregistry.org/Part:pSB2K3 pSB2K3]	FecA : oligo ordered but not received yet (10/08/09)	??	BBa_????
CC!	((rbs - fecA) :: ((Ompa Linker :: g3p) :: ter)) in [http://partsregistry.org/Part:pSB2K3 pSB2K3]	BBa_??? :: BBa_??? in [http://partsregistry.org/Part:pSB2K3 pSB2K3]		??	BBa_????
CC	((rbs - ClyA :: RFP) in [http://partsregistry.org/Part:pSB2K3 pSB2K3]	BBa_??? :: BBa_???? in [http://partsregistry.org/Part:pSB2K3 pSB2K3]	modif RFP (souf)- some troubles with amplification of clyA fusion C term (10/08/09)	??	BBa_????
CC	((rbs-RFP :: clyA) in [http://partsregistry.org/Part:pSB2K3 pSB2K3]	BBa_??? :: BBa_???? in [http://partsregistry.org/Part:pSB2K3 pSB2K3]	modif RFP (souf) - fusion N term de cly A (autre possibilité)- we still miss 1 primer for clyA N term fusion (10/08/09)	??	BBa_????
CC!	((rbs - ClyA :: RFP)) :: (((rbs - fecA) :: (rbs - jun/foss)) :: ter)) in [http://partsregistry.org/Part:pSB2K3 pSB2K3]	BBa_??? :: BBa_???? in [http://partsregistry.org/Part:pSB2K3 pSB2K3]	modif RFP (souf)	??	BBa_????
CC!	((rbs - ClyA :: RFP) :: (((rbs - fecA) :: (OmpA linker :: g3p)) :: ter)) in [http://partsregistry.org/Part:pSB2K3 pSB2K3]	BBa_??? :: BBa_???? in [http://partsregistry.org/Part:pSB2K3 pSB2K3]	modif RFP (souf)	??	BBa_????
CC!	(Pbad :: ((rbs - ClyA :: RFP) :: (((rbs - fecA) :: (OmpA linker :: g3p)) :: ter))) in [http://partsregistry.org/Part:pSB2K3 pSB2K3]	[http://partsregistry.org/Part:BBa_I0500 Bba_I0500] :: BBa_???? in [http://partsregistry.org/Part:pSB2K3 pSB2K3]		??	BBa_????
CC!	(Pbad :: ((rbs - ClyA :: RFP) :: (((rbs - fecA) :: (rbs - jun/foss)) :: ter))) in [http://partsregistry.org/Part:pSB2K3 pSB2K3]	BBa_??? :: BBa_???? in [http://partsregistry.org/Part:pSB2K3 pSB2K3]		??	BBa_????
CC!	((Pbad :: ((rbs - ClyA :: RFP) :: (((rbs - fecA) :: (OmpA linker :: g3p)) :: ter))) :: ((Pbad :: rbs - lacI - LVA) :: ter)) in [http://partsregistry.org/Part:pSB2K3 pSB2K3]	BBa_??? :: BBa_???? in [http://partsregistry.org/Part:pSB2K3 pSB2K3]	2Pbad et 2ter?? (souf)	??	BBa_????
CC!	((Pbad :: ((rbs - ClyA :: RFP) :: (((rbs - fecA) :: (rbs - jun/foss)) :: ter))) :: ((Pbad :: rbs - lacI - LVA) :: ter)) in [http://partsregistry.org/Part:pSB2K3 pSB2K3]	BBa_??? :: BBa_???? in [http://partsregistry.org/Part:pSB2K3 pSB2K3]		??	BBa_????

Plasmid B

Designer	Parts description	Corresponding BioBricks	Status (last update)	Plasmid #	Registry
GC	((Plac :: rbs-tetR) :: rbs-tolRII) in pSB2A3	BBa_blabla :: BBa_tralala in truc	Planning / oligo desgned or ordered / PCR in progress / cloned / sequencing... (10/08/09)	12	BBa_pouet pouet
CC	(Plac :: rbs-tetR) in [http://partsregistry.org/Part:pSB1A3 pSB1A3]	[http://partsregistry.org/Part:BBa_I14032 Bba_I14032] :: [http://partsregistry.org/Part:BBa_K136050 BBa_K136050] in [http://partsregistry.org/Part:pSB1A3 pSB1A3]	both have been digested and purified, ligation in progress (10/08/09)	??	BBa_????
CC	((Plac :: rbs-tetR) :: ter) in [http://partsregistry.org/Part:pSB1A3 pSB1A3]	BBa_???? :: [http://partsregistry.org/Part:BBa_B0014 BBa_B0014] in [http://partsregistry.org/Part:pSB1A3 pSB1A3]		??	[http://partsregistry.org/wiki/index.php?title=Part:BBa_K257015 BBa_K257015]
CC	(ter :: ((Plac :: rbs-tetR) :: ter)) in [http://partsregistry.org/Part:pSB1A3 pSB1A3]	[http://partsregistry.org/Part:BBa_B0014 BBa_B0014] :: [http://partsregistry.org/wiki/index.php?title=Part:BBa_K257015 BBa_K257015] in [http://partsregistry.org/Part:pSB1A3 pSB1A3]		??	BBa_????
CL	(rbs - OmpAsignal :: TolR II)	[http://partsregistry.org/wiki/index.php?title=Part:BBa_K257006 BBa_K257006] ::[http://partsregistry.org/wiki/index.php?title=Part:BBa_K257005 BBa_K257005] in [http://partsregistry.org/Part:pSB1A3 pSB1A3]	OmpA signal and TolRII amplified, but neither digested nor purified (10/08/09)	??	BBa_????
CC	(Ptet :: (rbs - OmpAsignal :: TolRII)) in [http://partsregistry.org/Part:pSB1A3 pSB1A3]	[http://partsregistry.org/Part:BBa_R0040 Bba_R0040] :: [http://partsregistry.org/wiki/index.php?title=Part:BBa_K257006 BBa_K257006] ::[http://partsregistry.org/wiki/index.php?title=Part:BBa_K257005 BBa_K257005] in [http://partsregistry.org/Part:pSB1A3 pSB1A3]	Ptet digested and purified (10/08/09)	??	BBa_????
CC	(rbs :: EYFP) in [http://partsregistry.org/Part:pSB1A3 pSB1A3]	BBa_??? :: [http://partsregistry.org/wiki/index.php?title=Part:BBa_E0030 BBa_E0030] in [http://partsregistry.org/Part:pSB1A3 pSB1A3]	y a t-il vraiment 2 biobricks ou bien met-on le RBS direct dans des oligos pour RFP? (souf) biobrick to find or primers to design (10/08/09)	??	BBa_????
CC	(Ptet :: (rbs :: EYFP)) in [http://partsregistry.org/Part:pSB1A3 pSB1A3]	[http://partsregistry.org/Part:BBa_R0040 Bba_R0040] :: BBa_??? in [http://partsregistry.org/Part:pSB1A3 pSB1A3]		??	BBa_????
CC	((Ptet :: (rbs :: EYFP)) :: (ter :: ((Plac :: rbs-tetR) :: ter))) in [http://partsregistry.org/Part:pSB1A3 pSB1A3]	BBa_??? :: BBa_??? in [http://partsregistry.org/Part:pSB1A3 pSB1A3]		??	BBa_????
CC	((Ptet :: (rbs - OmpAsignal :: TolRIIP)) :: (ter :: ((Plac :: rbs-tetR) :: ter))) in [http://partsregistry.org/Part:pSB1A3 pSB1A3]	BBa_??? :: BBa_??? in [http://partsregistry.org/Part:pSB1A3 pSB1A3]		??	BBa_????

Plasmid C

Designer	Parts description	Corresponding BioBricks	Status (last update)	Plasmid #	Registry
CC	(rbs-FecI :: rbs-FecR) in [http://partsregistry.org/Part:pSB2K3 pSB2K3]	BBa_??? :: BBa_???	primers ordered but not received yet (10/08/09)	??	BBa_????
CC	PfecA in [http://partsregistry.org/Part:BBa_J61002 Bba_J61002]	BBa_???	vector digested and purified, Pfec A amplified but not digested (purified?) (10/08/09)	??	BBa_????