Team:UNICAMP-Brazil/Notebooks/August 10

ColiGuard

Recovering the BioBricks

Today we ressuspended the chosen BioBricks according to the standard protocol. Each undergraduated student was responsible to ressuspend a set of BioBricks. All of them were transfomed into electrocompetent E. coli (DH10B) according to Protocol 3 (see protocols section). The cells were then plated in LB media containing the appropriate antibiotic.

Marcelo

Part:BBa_B0030 - RBS.1 (strong) -- modified from R. Weiss

Part:BBa_B0032 - RBS.3 (medium) -- derivative of BBa_0030

Luige

Part:BBa_J61100 - Ribosome Binding Site Family Member

Part:BBa_J61107 - Ribosome Binding Site Family Member

Marcos

Part:BBa_B0010 - T1 from E. coli rrnB

Part:BBa_B0014 - double terminator

Leonardo

Part:BBa_B0015 - double terminator (B0010-B0012)

Part:BBa_R0040 - TetR repressible promoter

Part:BBa_J23100 - constitutive promoter family member

Part:BBa_J23107 - constitutive promoter family member

Fabiana

Part:BBa_J23109 - constitutive promoter family member

Part:BBa_J31007 - tetracycline resistance protein TetA(C)

Part:BBa_J31003 - kanamycin resistance forward

Part:BBa_E0040 - green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212)

Victor

Part:BBa_E1010: **highly** engineered mutant of red fluorescent protein from Discosoma striata

Part:pSB1A3 - High copy BioBrick assembly plasmid

Part:pSB1AK3 - High copy BioBrick assembly plasmid

Part:pSB3K3 - Low to medium copy BioBrick standard vector

Part:pSB6A1 - pBR322 converted to BioBrick vector (formally BBa_I739201)