Back to Calendar
YeastGuard
Gallus gallus RNA
Quantification
We have verified the concentration of RNA we got in the RNA extractions from the G. gallus organs. The following table shows the results.
| Samples | ng/uL | 260/280 | 260/230
|
| Blood 1 | 743.42 | 2.01 | 1.78
|
| Blood 2 | 604.84 | 1.99 | 1.54
|
| Blood 3 | 1814.10 | 1.97 | 1.88
|
| Spleen 1 | 3607.90 | 1.96 | 1.57
|
| Spleen 2 | 3715.95 | 1.94 | 1.55
|
| Spleen 3 | 4213.93 | 1.78 | 1.69
|
| Spleen 4 | 4243.37 | 1.76 | 1.69
|
| Oviduct 1 | 4362.04 | 1.66 | 1.41
|
| Oviduct 2 | 3498.69 | 1.87 | 1.16
|
| Oviduct 3 | 3601.43 | 1.84 | 1.13
|
| Oviduct 4 | 3568.66 | 1.84 | 1.12
|
The concentration values are ok, but the ratios 260/280 and 260/230 are not that good. Let’s find out what we see in the agarose gel.
Raíssa
Agarose gel
Taís
Terminator Biobrick
Today we perfomed miniprep of the Terminator Biobrick transformed E. coli, according to Protocol 2 (see protocols section).
Wesley
|
"
