- Few colonies were found this morning =( The test wasn't conclusive so we decided to go on with the SAP dephosphorylation protocol (protocol D).
New biobricks
- Today we performed 5' dephosphorylation of the biofusion vector with SAP (Protocol SAP). We did the ligation reaction (Protocol D) with the 5' dephosphorylated vector and the three digested (XbaI and SpeI)new parts (pJen1, pDLD and Lysozyme).
- We transformed the thermocompetent E. coli (protocol A) with the ligations and plated in LB+Amp media.
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