After the O/N period, today we transformed the ligation finO+pSB1A3 into electrocompetent E. coli bacteria, strain DH10B. We followed protocol 3, without modifications (see Protocols section).
We then plated the transfomed cells in LB-AMP media, and let them grow at 37ºC for an O/N period.
finP+pSB1A3
We followed the same produce for the finP+pSB1A3 ligation.
Marcelo
YeastGuard
New biobrick - The first screening
6 colonies of each new biobrick were chosen of the plates made yesterday to grow in liquid LB.
Then, we performed miniprep to get our new biobricks purified (Protocol 2).
We then digested the plasmids obtained by the miniprep with XbaI to confirm the correct ligation of the parts following the biobrick format (Protocol 11). However, the electrophoresis gel (shown below) showed an unexpected pattern of bands that can't confirm correctly ligations of our biobricks. =(
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