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Team:Paris/constructions
From 2009.igem.org
iGEM > Paris > Freezer > Constructions in progress
Constructions
| Color code for sticker | |||||||
|---|---|---|---|---|---|---|---|
| Vector | Insert | PCR product | Plasmid | Strains | Kanamycin | Ampicilin | Chloranphenicol |
- Plasmid A
| Designer | Parts description | Corresponding BioBricks | Status (last update) | Plasmid # | Registry |
| CC | (Pbad :: rbs - lacI - LVA) in [http://partsregistry.org/Part:pSB1A3 pSB1A3]???Pbad is on PSB2K3 | [http://partsregistry.org/Part:BBa_I0500 Bba_I0500] :: [http://partsregistry.org/Part:BBa_J24679 BBa_J24679] in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | pSB1A3 transformed, digested, PBad transformed and not digested well- LacI transformed and digested (10/08/09) | ?? | BBa_???? |
| CC | (Pbad :: rbs - lacI - LVA) :: ter in [http://partsregistry.org/Part:pSB1A3 pSB1A3] | BBa_??? :: [http://partsregistry.org/Part:BBa_B0014 BBa_B0014] in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | ter transformed, digested and purified (10/08/09) | ?? | BBa_???? |
| CC | ((Pbad :: rbs - lacI - LVA) :: RFP) in [http://partsregistry.org/Part:pSB1A3 pSB1A3] | BBa_??? :: [http://partsregistry.org/wiki/index.php?title=Part:BBa_K093005 BBa_K093005] in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | pSB2K3 transformed and digested, RFP : primers designed but not yet ordered (10/08/09) | ?? | BBa_????
|
| CC | (((Pbad :: rbs - lacI - LVA) :: RFP) :: ter) in [http://partsregistry.org/Part:pSB1A3 pSB1A3] | BBa_??? :: [http://partsregistry.org/Part:BBa_B0014 BBa_B0014] in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | ?? | BBa_????
| |
| CC | (OmpA Linker :: g3p) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | [http://partsregistry.org/Part:BBa_K103006 BBa_K103006] :: BBa_??? in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | OmpA linker standardisé - OmpA amplified, not purified - Same for g3p (10/08/09) | ?? | BBa_???? |
| (g3p :: clyA) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | BBa_??? :: BBa_??? in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | autre possibilité (souf)- 1 primer missing for C term fusion with clyA (10/08/09) | ?? | BBa_???? | |
| CC! | ((OmpA Linker :: g3p) :: ter) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | BBa_??? :: [http://partsregistry.org/Part:BBa_B0014 BBa_B0014] in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | ?? | BBa_???? | |
| CC! | ((???autotransporter??? - jun/foss) :: ter) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | BBa_??? :: [http://partsregistry.org/Part:BBa_B0014 BBa_B0014] in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | jun/foss has to be synthetized & autotransporter to be found!!!! (10/08/09) | ?? | BBa_???? |
| CC! | ((rbs - fecA) :: ((rbs - jun/foss) :: ter)) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | BBa_??? :: BBa_??? in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | FecA : oligo ordered but not received yet (10/08/09) | ?? | BBa_???? |
| CC! | ((rbs - fecA) :: ((Ompa Linker :: g3p) :: ter)) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | BBa_??? :: BBa_??? in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | ?? | BBa_???? | |
| CC | ((rbs - ClyA :: RFP) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | BBa_??? :: BBa_???? in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | modif RFP (souf)- some troubles with amplification of clyA fusion C term (10/08/09) | ?? | BBa_???? |
| CC | ((rbs-RFP :: clyA) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | BBa_??? :: BBa_???? in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | modif RFP (souf) - fusion N term de cly A (autre possibilité)- we still miss 1 primer for clyA N term fusion (10/08/09) | ?? | BBa_???? |
| CC! | ((rbs - ClyA :: RFP)) :: (((rbs - fecA) :: (rbs - jun/foss)) :: ter)) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | BBa_??? :: BBa_???? in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | modif RFP (souf) | ?? | BBa_???? |
| CC! | ((rbs - ClyA :: RFP) :: (((rbs - fecA) :: (OmpA linker :: g3p)) :: ter)) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | BBa_??? :: BBa_???? in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | modif RFP (souf) | ?? | BBa_???? |
| CC! | (Pbad :: ((rbs - ClyA :: RFP) :: (((rbs - fecA) :: (OmpA linker :: g3p)) :: ter))) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | [http://partsregistry.org/Part:BBa_I0500 Bba_I0500] :: BBa_???? in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | ?? | BBa_???? | |
| CC! | (Pbad :: ((rbs - ClyA :: RFP) :: (((rbs - fecA) :: (rbs - jun/foss)) :: ter))) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | BBa_??? :: BBa_???? in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | ?? | BBa_???? | |
| CC! | ((Pbad :: ((rbs - ClyA :: RFP) :: (((rbs - fecA) :: (OmpA linker :: g3p)) :: ter))) :: ((Pbad :: rbs - lacI - LVA) :: ter)) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | BBa_??? :: BBa_???? in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | 2*Pbad et 2*ter?? (souf) | ?? | BBa_???? |
| CC! | ((Pbad :: ((rbs - ClyA :: RFP) :: (((rbs - fecA) :: (rbs - jun/foss)) :: ter))) :: ((Pbad :: rbs - lacI - LVA) :: ter)) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | BBa_??? :: BBa_???? in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | ?? | BBa_????
|
- Plasmid B
| Designer | Parts description | Corresponding BioBricks | Status (last update) | Plasmid # | Registry |
| GC | ((Plac :: rbs-tetR) :: rbs-tolRII) in pSB2A3 | BBa_blabla :: BBa_tralala in truc | Planning / oligo desgned or ordered / PCR in progress / cloned / sequencing... (10/08/09) | 12 | BBa_pouet pouet |
| CC | (Plac :: rbs-tetR) in [http://partsregistry.org/Part:pSB1A3 pSB1A3] | [http://partsregistry.org/Part:BBa_I14032 Bba_I14032] :: [http://partsregistry.org/Part:BBa_K136050 BBa_K136050] in [http://partsregistry.org/Part:pSB1A3 pSB1A3] | both have been digested and purified, ligation in progress (10/08/09) | ?? | BBa_???? |
| CC | ((Plac :: rbs-tetR) :: ter) in [http://partsregistry.org/Part:pSB1A3 pSB1A3] | BBa_???? :: [http://partsregistry.org/Part:BBa_B0014 BBa_B0014] in [http://partsregistry.org/Part:pSB1A3 pSB1A3] | ?? | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K257015 BBa_K257015] | |
| CC | (ter :: ((Plac :: rbs-tetR) :: ter)) in [http://partsregistry.org/Part:pSB1A3 pSB1A3] | [http://partsregistry.org/Part:BBa_B0014 BBa_B0014] :: [http://partsregistry.org/wiki/index.php?title=Part:BBa_K257015 BBa_K257015] in [http://partsregistry.org/Part:pSB1A3 pSB1A3] | ?? | BBa_???? | |
| CL | (rbs - OmpAsignal :: TolR II) | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K257006 BBa_K257006] ::[http://partsregistry.org/wiki/index.php?title=Part:BBa_K257005 BBa_K257005] in [http://partsregistry.org/Part:pSB1A3 pSB1A3] | OmpA signal and TolRII amplified, but neither digested nor purified (10/08/09) | ?? | BBa_???? |
| CC | (Ptet :: (rbs - OmpAsignal :: TolRII)) in [http://partsregistry.org/Part:pSB1A3 pSB1A3] | [http://partsregistry.org/Part:BBa_R0040 Bba_R0040] :: [http://partsregistry.org/wiki/index.php?title=Part:BBa_K257006 BBa_K257006] ::[http://partsregistry.org/wiki/index.php?title=Part:BBa_K257005 BBa_K257005] in [http://partsregistry.org/Part:pSB1A3 pSB1A3] | Ptet digested and purified (10/08/09) | ?? | BBa_???? |
| CC | (rbs :: EYFP) in [http://partsregistry.org/Part:pSB1A3 pSB1A3] | BBa_??? :: [http://partsregistry.org/wiki/index.php?title=Part:BBa_E0030 BBa_E0030] in [http://partsregistry.org/Part:pSB1A3 pSB1A3] | y a t-il vraiment 2 biobricks ou bien met-on le RBS direct dans des oligos pour RFP? (souf) biobrick to find or primers to design (10/08/09) | ?? | BBa_???? |
| CC | (Ptet :: (rbs :: EYFP)) in [http://partsregistry.org/Part:pSB1A3 pSB1A3] | [http://partsregistry.org/Part:BBa_R0040 Bba_R0040] :: BBa_??? in [http://partsregistry.org/Part:pSB1A3 pSB1A3] | ?? | BBa_???? | |
| CC | ((Ptet :: (rbs :: EYFP)) :: (ter :: ((Plac :: rbs-tetR) :: ter))) in [http://partsregistry.org/Part:pSB1A3 pSB1A3] | BBa_??? :: BBa_??? in [http://partsregistry.org/Part:pSB1A3 pSB1A3] | ?? | BBa_???? | |
| CC | ((Ptet :: (rbs - OmpAsignal :: TolRIIP)) :: (ter :: ((Plac :: rbs-tetR) :: ter))) in [http://partsregistry.org/Part:pSB1A3 pSB1A3] | BBa_??? :: BBa_??? in [http://partsregistry.org/Part:pSB1A3 pSB1A3] | ?? | BBa_???? |
- Plasmid C
| Designer | Parts description | Corresponding BioBricks | Status (last update) | Plasmid # | Registry |
| CC | (rbs-FecI :: rbs-FecR) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | BBa_??? :: BBa_??? | primers ordered but not received yet (10/08/09) | ?? | BBa_????
|
| CC | PfecA in [http://partsregistry.org/Part:BBa_J61002 Bba_J61002] | BBa_??? | vector digested and purified, Pfec A amplified but not digested (purified?) (10/08/09) | ?? | BBa_???? |
