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EPF-Lausanne/18 August 2009
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==Wet Lab== | ==Wet Lab== | ||
| + | Miniprep of RBS and our LacI-RBS | ||
| + | Colony PCR of different LovTap-Term and digestion assay by Pst1. Result after an agarose gel: All colonies seem to have the LovTap part in a plasmid (the LovTap has two P sites). | ||
| + | |||
| + | We re-did a 1.5 step PCR of the T7, LacLP and LacI biobricks and keep the products for the next day. | ||
| + | |||
| + | We did a PCR of T7-CBP-LovTap-Term, a digestion, a ligation and transformation into a E1010 backbone. | ||
| + | |||
| + | Growing cells with RBS, LacI-RBS, 5 clones of the second "Readout 2" plasmids in a LB medium | ||
| + | |||
| + | Growing cells with the first "Readout 2" plasmid of 4 clones in a M9 medium without Tryptophan. | ||
==People in the lab== | ==People in the lab== | ||
| - | Nath, Basile, Gab, Christian | + | Nath, Basile, Gab, Christian, Heidi |
Latest revision as of 09:16, 20 August 2009
Contents |
Wet Lab
Miniprep of RBS and our LacI-RBS
Colony PCR of different LovTap-Term and digestion assay by Pst1. Result after an agarose gel: All colonies seem to have the LovTap part in a plasmid (the LovTap has two P sites).
We re-did a 1.5 step PCR of the T7, LacLP and LacI biobricks and keep the products for the next day.
We did a PCR of T7-CBP-LovTap-Term, a digestion, a ligation and transformation into a E1010 backbone.
Growing cells with RBS, LacI-RBS, 5 clones of the second "Readout 2" plasmids in a LB medium
Growing cells with the first "Readout 2" plasmid of 4 clones in a M9 medium without Tryptophan.
People in the lab
Nath, Basile, Gab, Christian, Heidi
