IBB Pune/21 June 2009

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(New page: I) Transformation with PCR product, dilute DNA 1:10 and use 4ul. NO Transformants :-( Why? * Dilution? * Linear DNA, try ligation before transformation. * Kan conc too high? II) Ligat...)
 
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Transformation with PCR product, dilute DNA 1:10 and use 4ul.
Transformation with PCR product, dilute DNA 1:10 and use 4ul.

Latest revision as of 13:56, 21 October 2009




I) Transformation with PCR product, dilute DNA 1:10 and use 4ul.

NO Transformants  :-(

Why?

  • Dilution?
  • Linear DNA, try ligation before transformation.
  • Kan conc too high?


II) Ligation test. RE digest of pBR322 and pGFP, gel and ligation.