Team:Calgary/14 August 2009

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University of Calgary

UNIVERSITY OF CALGARY



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AUGUST 14, 2009


CAROL

Descriptive Title of What You’re Doing

WIKI CODING HERE

CHINMOYEE

Descriptive Title of What You're Doing

WIKI CODING HERE


EMILY

Descriptive Title of What You're Doing

WIKI CODING HERE


FAHD

Descriptive Title of What You're Doing

WIKI CODING HERE


IMAN

Membrane Computing Current Status: Modifying the engine (Gillespie's Algorithm)!

Working on second version of Gillespie's algorithm - adding ability to apply rules in parallel. Also working on our Paper (now in LaTex!).


JAMIE

AI-2 Activity Assay

Cell free supernants from DH5a, S. typhimurium and V. harveyi were made from overnight cultures and were tested with various reporter strains. A plate reader was used to take luminescence readings for a period of 24 hours.

JEREMY

Descriptive Title of What You're Doing

WIKI CODING HERE


KATIE

Descriptive Title of What You're Doing

WIKI CODING HERE


KEVIN

Verifying the fluorescence of the cells with reporter and mutant circuit

The cells with reporter and mutant circuits were viewed under UV in order to see if the mutants and the reporter circuits are properly functional. However, no colonies were found to be glowing. Since the level of GFP production is low due to the inducible low copy plasmid that it is in, we may have to grow an overnight culture and measure the fluorescence of the culture with the plate reader. If the fluorescence is found to be too low to decide whether or not the circuits are properly functioning, we may have to induce the cells with IPTG to induce the duplication of the reporter circuit. This would hopefully increase the production levels of GFP because more Pqrr4 promoters would be available for the mutant proteins to attach to.

MANDY

Descriptive Title of What You're Doing

WIKI CODING HERE


PATRICK

Friday: Hard Work Pays Off!

Feast your eyes on this:

Calgary-Biobricker UI.png

The Biobricker portion of my Second Life work is officially almost done!

Calgary-Biobricker zoom.png

Most of my work today was chasing down the last few bugs in the assembly system. It can now build any biobrick device you can dream of, in game right before your eyes. Obviously, you will be able to build more than nonsense parts consisting of just terminators and promoters. I'm presently in the business of uploading all of the fantastic little icons Mandy has made to replace the letters standing in for buttons, incorporating them into the UI will be a pretty big cut and paste job though! Once they're all uploaded, I'll generate the first batch of physical parts, bundle them all together, and make the first ever release of the Biobrick UI to the other SLers for testing.

The Biobricker, in brief. The top row of buttons access the various biobrick parts encoded in the UI, with a constituitive repressor and terminator being the only examples right now. The other buttons open up menus, leading to activatable and repressable promoters, coding sequences, and operator sites.

The bottom row of buttons gives you control over the prospective device. Of particular importance is the 'B' button, which is where you'll click to build your device. The UI also supports deleting and inserting parts in the biobrick, permitting easy experimentation.

I had some doubts about just how useful and interesting the biobricker would actually be... but It's super exciting to see all the pieces coming together and working nicely!

Most of the rest of today was consumed with a presentation on our Fort MacMurray oilsands tour, as well as an intense question and answer drill, in preparation for the jamboree.



PRIMA

Descriptive Title of What You're Doing

WIKI CODING HERE


STEFAN

Descriptive Title of What You're Doing

WIKI CODING HERE


VICKI

Descriptive Title of What You're Doing

WIKI CODING HERE