Team:Calgary/16 July 2009

From 2009.igem.org

(Difference between revisions)
Line 172: Line 172:
| ''sig70-F2  || 1.55 || 1.80 || 44284 || 17713.6 || 1277.91 || 3.1 || 46.9
| ''sig70-F2  || 1.55 || 1.80 || 44284 || 17713.6 || 1277.91 || 3.1 || 46.9
|-
|-
-
| ''psB1AC3 3  || 1.87|| 1.88|| 48.5
+
| ''sig70-F3 || 1.62 || 1.86 || 41323||16529.2||1192.46||3.4||46.6
|-
|-
-
| ''psB1AC3 4|| 1.90|| 2.11|| 118.9
+
| ''sig70-F4 || 1.70 || 1.99 ||32365||12946||933.96||4.3||45.7
|-
|-
-
| ''psB1AK3 1 || 2.05 || 2.42 || 134.4
+
| ''sig70-F5 || 1.72 || 2.07 ||33688||13475.2||972.14||4.1|45.9
|-
|-
-
| ''psB1AK3 2 || 1.95 || 2.23 || 55.6
+
| ''sig70-F6 ||2.07 ||1.95||45412||18164.8||1310.46||3.1||46.9
|-
|-
-
| ''psB1AK3 3  || 1.91 || 2.22 || 97.9
+
| ''sig70-F7 || 1.64 || 1.95 ||39046||15618.4||1126.76||3.6||46.4
|-
|-
-
| ''psB1AK3 4 || 2.03 || 1.92 || 44.5
+
| ''sig70-F8 || 1.43 ||1.63 ||45745||18298||1320.07||3.0||47.0
 +
|-
 +
| ''sig70-F9 || 1.75 ||2.16 ||23628||9451.2||681.84||5.9||44.1
 +
|-
 +
| ''sig70-F10 || 1.72 ||2.01||32682||13072.8||943.11||4.2||45.8
 +
|-
 +
| ''sig70-F11 || 1.71 ||2.07||31494||12597.6||908.83||4.4||45.6
 +
|-
 +
| ''sig70-F12 || 1.59 || 1.82 || 41202||16480.8||1188.97||3.4||46.6
 +
|-
 +
| ''sig70-F13 || 1.73 || 2.03 ||29224||11689.6||843.3||4.7||45.3
 +
|-
 +
| ''sig70-F14 ||1.41||1.58 ||45645||18258||1317.2||3.0||47.0
 +
|-
 +
| ''sig70-F15 || 1.59 || 1.85 || 40681||16272.4||1173.9||3.4||46.6
 +
|-
 +
| ''sig70-F16 || 1.57 || 1.82 || 42241||16896.4||1219.0||3.3||46.7
 +
|-
 +
| ''sig70-R1 || 2.06 ||2.17 ||7516||3006.4||216.9||18.4||31.6
 +
|-
 +
| ''sig70-R2 ||2.04 ||2.25 ||15255||6102.0||440.2||9.1||40.9
 +
|-
 +
| ''sig70-R3 ||1.76 || 1.85 ||42503||17001.2||1226.5||3.3||46.7
 +
|-
 +
| ''sig70-R4||2.01||2.21||24012||9604.8||692.9||5.8||44.2
 +
|-
 +
| ''sig70-R5||2.10||2.27||18530||7412||534.7||7.5||42.5
 +
|-
 +
| ''sig70-R6||1.98||2.19||28452||11380.8||821.0||4.9||45.1
 +
|-
 +
| ''sig70-R7 || 1.94||2.17||32976||13190.4||951.6||4.2||45.8
 +
|-
 +
| ''sig70-R8 ||2.00||2.20||25379||10151.6||732.4||5.5||44.5
 +
|-
 +
| ''sig70-R9 || 2.00 ||2.24 ||24141||9656.4||696.6||5.9||44.1
 +
|-
 +
| ''sig70-R10 ||1.94 ||2.17 ||30237||12094.8||872.6||4.6||45.4
 +
|-
 +
| ''sig70-R11 || 1.92||2.16 ||31497||12598.8||908.9||4.4||45.6
 +
|-
 +
| ''sig70-R12 || 1.92 || 2.17 ||30045||12018||867.0||4.6||45.4
 +
|-
 +
| ''sig70-R13 ||2.01||2.18||18726||7490.4||540.4||7.4||42.6
 +
|-
 +
| ''sig70-R14 || 1.88 ||2.03||36111||14444.4||1042.1||3.8||46.2
 +
|-
 +
| ''sig70-R15 ||1.98 ||2.17||10679||4271.6||308.2||13||37
 +
|-
 +
| ''sig70-R16 || 1.86 ||2.01 ||35969||14387.6||1038||3.9||46.1
|}
|}
</center>
</center>

Revision as of 19:16, 29 July 2009

University of Calgary

UNIVERSITY OF CALGARY



THIS MONTH

July
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30 31


NOTEBOOK PAGE INDEX
Carol
Chinmoyee
Emily
Fahd
Iman
Jamie
Jeremy
Katie
Kevin
Mandy
Patrick
Prima
Stefan
Vicki



CALENDAR

May
MTWTFSS
        1 2 3
4 5 6 7 8 9 10
11 12 13 14 15 16 17
18 19 20 21 22 23 24
25 26 27 28 29 30 31


June
MTWTFSS
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30


July
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30 31


August
MTWTFSS
          1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31


September
MTWTFSS
  1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30


October
MTWTFSS
      1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31



JULY 16, 2009


CAROL

Diluting Primers and Primer Extension Polymerase Chain Reaction (PCR)

  • To create the synthetic sigma 70 promoter library, we have synthesized 32 different primers (16 different forward primers and 16 different reverse primers) to hopefully form 256 different promoters.
  • Need to dilute primers to 80μM. The following is the concentration of the original primers that were synthesized once water was added to the primers.
Primer 260/280 260/230 Concentration [ng/μL] Reading/2.5 [ng/μL] Stock Concentration [μM] Volume stock into working [μL] Volume water into working [μL]
sig70-F1 1.98 1.73 20816 832.64 60.07 6.7 43.3
sig70-F2 1.55 1.80 44284 17713.6 1277.91 3.1 46.9
sig70-F3 1.62 1.86 4132316529.21192.463.446.6
sig70-F4 1.70 1.99 3236512946933.964.345.7
sig70-F5 1.72 2.07 3368813475.2972.1445.9
sig70-F6 2.07 1.954541218164.81310.463.146.9
sig70-F7 1.64 1.95 3904615618.41126.763.646.4
sig70-F8 1.43 1.63 45745182981320.073.047.0
sig70-F9 1.75 2.16 236289451.2681.845.944.1
sig70-F10 1.72 2.013268213072.8943.114.245.8
sig70-F11 1.71 2.073149412597.6908.834.445.6
sig70-F12 1.59 1.82 4120216480.81188.973.446.6
sig70-F13 1.73 2.03 2922411689.6843.34.745.3
sig70-F14 1.411.58 45645182581317.23.047.0
sig70-F15 1.59 1.85 4068116272.41173.93.446.6
sig70-F16 1.57 1.82 4224116896.41219.03.346.7
sig70-R1 2.06 2.17 75163006.4216.918.431.6
sig70-R2 2.04 2.25 152556102.0440.29.140.9
sig70-R3 1.76 1.85 4250317001.21226.53.346.7
sig70-R42.012.21240129604.8692.95.844.2
sig70-R52.102.27185307412534.77.542.5
sig70-R61.982.192845211380.8821.04.945.1
sig70-R7 1.942.173297613190.4951.64.245.8
sig70-R8 2.002.202537910151.6732.45.544.5
sig70-R9 2.00 2.24 241419656.4696.65.944.1
sig70-R10 1.94 2.17 3023712094.8872.64.645.4
sig70-R11 1.922.16 3149712598.8908.94.445.6
sig70-R12 1.92 2.17 3004512018867.04.645.4
sig70-R13 2.012.18187267490.4540.47.442.6
sig70-R14 1.88 2.033611114444.41042.13.846.2
sig70-R15 1.98 2.17106794271.6308.21337
sig70-R16 1.86 2.01 3596914387.610383.946.1


CHINMOYEE

Descriptive Title of What You're Doing

WIKI CODING HERE


EMILY

Descriptive Title of What You're Doing

WIKI CODING HERE


FAHD

Descriptive Title of What You're Doing

WIKI CODING HERE


IMAN

Descriptive Title of What You're Doing

WIKI CODING HERE


JAMIE

Descriptive Title of What You're Doing

WIKI CODING HERE


JEREMY

Descriptive Title of What You're Doing

WIKI CODING HERE


KATIE

Descriptive Title of What You're Doing

WIKI CODING HERE


KEVIN

Descriptive of What You're Doing

WIKI CODING HERE


MANDY

Descriptive Title of What You're Doing

WIKI CODING HERE


PATRICK

Descriptive Title of What You're Doing

WIKI CODING HERE


PRIMA

Descriptive Title of What You're Doing

WIKI CODING HERE


STEFAN

Descriptive Title of What You're Doing

WIKI CODING HERE


VICKI

Descriptive Title of What You're Doing

WIKI CODING HERE

Retrieved from "http://2009.igem.org/Team:Calgary/16_July_2009"