Team:Calgary/17 August 2009

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University of Calgary

UNIVERSITY OF CALGARY



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AUGUST 17, 2009


CAROL

Descriptive Title of What You’re Doing

WIKI CODING HERE

CHINMOYEE

Working with MATLAB Day1

Researched Sensitivity Analysis:

Intro into sensitivity Analysis , by Lucia Breierova and Mark Choudhari...

Content:

Examples / Tutorial on how to do sensitivity analysis on data ( No examples related to our work)

Learned:

Sensitivity investigates the robustness of a study when the study includes some form of math modelling. Sensitivity allows the modeller to determine what level of accuracy is needed for a parameter to make the model sufficiently useful and valid. When the parameter is insensitive in the model , making an estimation is a good idea.

With Systems Dynamic models the k parameter doesn't affect the behaviour of the graph . The shape of the graph stays the same .

Matlab Programing:

Looked into the effect of changing the kinetic constants on the graph. This resulted in no change in the behavior of the graph as predicted by the paper. The max/min/ steepness of the graphs changed as a result of changing k values. The change of some k values effected the steepness of the lines very much while changing the value of other k values didn't change the steepeness all that much.

Also looked into the effect of changing the initial concentration of the species . This completely changed the behavior of the graphs.

Looked into sensitivity analysis with simbiology interface . Still trying to work out some kinks.


EMILY

Transformation of J13002-LuxOD47A-B0015 into TOP10 cells, restreaks and overnight cultures of J13002-LuxOD47E-B0015 in KT1144 cells

  • This morning I made a detailed lab plan of what I need to do for the week. I also spent some time updating my lab notebook as well as the Wiki Notebook.
  • This afternoon I ligated J13002-LuxOD47A-B0015 and the psB1AC3 vector. I then transformed the ligated product into TOP10 cells and plated on KC plates and left for overnight growth.
  • I also did restreaks of my construct (J13002-LuxOD47E-B0015) in KT1144 cells on KC plates. I also prepared overnight cultrues of selected colonies in KC broth. Tomorrow Kevin and I will be using the plate reader to test these cultures for glow.



FAHD

Descriptive Title of What You're Doing

WIKI CODING HERE


IMAN

Working on Paper

I am gathering information needed to write down the paper we have been asked to write. I have written some sections so far.


JAMIE

Descriptive Title of What You're Doing

WIKI CODING HERE


JEREMY

Descriptive Title of What You're Doing

WIKI CODING HERE


KATIE

Descriptive Title of What You're Doing

WIKI CODING HERE


KEVIN

Descriptive of What You're Doing

WIKI CODING HERE


MANDY

Descriptive Title of What You're Doing

WIKI CODING HERE


PATRICK

Descriptive Title of What You're Doing

WIKI CODING HERE


PRIMA

Colony PCR and Marketing

It turns out that I had used the Low-melt agarose on Friday to make my 1% gel. Unfortunately, the gel didn't turn out well and I had to postpone my work to Monday. Today, I made a 1% gel with the normal agarose powder and remade my stock (3 microL PCR product, 2 microL dye & 15 microL ddH2O) and ran my gel at 100V. The results showed exactly what I was expecting. Since I had used regular BBK CP primers and length of my construct was 885bp, the total length of the bands should've been 1135bp. My bands were just above the 1kb ladder band! I let the students visiting our lab make my overnight cultures. Tomorrow, I'll do a mini-prep and a restriction digest to verify the size of my construct. If all else is good, I'll send it to sequencing.

I followed up with the T-shirt company but unfortunately I couldn't get a hold of her. I'll follow up tomorrow. I also called numerous new companies and sent our sponsorship package to one of them. I left a voice mail for the rest of the companies. I'll follow up with them tomorrow. I also began write-ups for the August newsletter.


STEFAN

Descriptive Title of What You're Doing

WIKI CODING HERE


VICKI

Descriptive Title of What You're Doing

WIKI CODING HERE