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CAROL
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WIKI CODING HERE
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CHINMOYEE
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EMILY
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- Purpose: To verify that BBK LuxOD47E has been successfully cloned into the psB1AC3 vector.
- Performed a Colony PCR on LuxOD47E in psB1AC3 vector colonies from last week. Only digests done with XbaI / PstI enzymes produced colonies on plates, so these were the only colonies used.
- Used p Taq and LuxO forward and reverse primers. Cycling conditions were as follows: 94 C for 6 minutes, 36x (94 C for 30 sec, 55 C for 45 sec, 72 C for 90 sec), 72 C for 10 minutes and hold at 4 C.
- See gel photo below. Lanes 1-6 are LuxOD47E cut with XbaI / PstI, colonies 1-6, Lane 7 is a ngeative control with ddH2O.
- Results: Nothing was amplified. This was because I used the wrong buffer. Will proceed by re-running the Colony PCR with the CORRECT buffer (10x PCR Buffer- Cl2).
- Performed colony PCR again with the proper PCR Buffer this time. PCR products were visualized on a 1% agarose gel run at 120 V with Generuler 1.0 KB+ DNA Ladder. Lanes 1-7 are LuxOD47E in psB1AC3 cut with XbaI / PstI. Lane 8 is a negative control with only Mastermix + ddH2O.
- See gel photo below.
- From this gel, it looks like LuxOD47E is in the vector as we see the approproate band size of approximately 1.4 KB.
- Prepared overnight cultures of the colonies, will isolate plasmid tomorrow.
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FAHD
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IMAN
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JAMIE
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JEREMY
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KATIE
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KEVIN
Restreak
Restreak of R0040, B0015, and J13002 were done to grow more of verified single colonies.
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MANDY
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PATRICK
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PRIMA
First Fundraising Event
This was iGEM Calgary's first bake sale. The team divided up the task of baking goodies. We met up at Emily's house yesterday to bake cupcakes, muffins, cakes and much more. We also had cinnamon buns, cheesecakes, samosas, cookies and the list goes on. we successfully raised $286.21.
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STEFAN
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VICKI
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