From 2009.igem.org
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Revision as of 05:51, 21 August 2009
University of Calgary
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CAROL
Descriptive Title of What You’re Doing
WIKI CODING HERE
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CHINMOYEE
CLASS
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EMILY
- Performed colony PCR again with the proper PCR Buffer this time. PCR products were visualized on a 1% agarose gel run at 120 V with Generuler 1.0 KB+ DNA Ladder. Lanes 1-7 are LuxOD47E in psB1AC3 cut with XbaI / PstI. Lane 8 is a negative control with only Mastermix + ddH2O.
- See gel photo below.
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FAHD
Descriptive Title of What You're Doing
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IMAN
Descriptive Title of What You're Doing
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JAMIE
Descriptive Title of What You're Doing
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JEREMY
Descriptive Title of What You're Doing
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KATIE
Descriptive Title of What You're Doing
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KEVIN
1. Glycerol Stock
Glycerol stocks of R0040, B0015 J13002, and Pqrr4 were made in order to preserve them long term. The addition of glycerl is important because otherwise the cellular membrane would shear.
2. Plasmid Isolation
To obtain more isolated R0040, B0015, J1302, and Pqrr4, miniprep (plasmid isolation) was done on them.
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MANDY
Descriptive Title of What You're Doing
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PATRICK
Descriptive Title of What You're Doing
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PRIMA
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STEFAN
Descriptive Title of What You're Doing
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VICKI
Sequencing of what I hope will be LuxOD47A BBK
I prepared a sample of the recently-formed-and-colony-PCR'd-and-NotI-restriction-digested LuxOD47A BBk for sequencing. This was done with BBk sequencing primers, in accordance with the sequencing protocol.
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