Team:Calgary/26 May 2009

From 2009.igem.org

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Sigma Genelute Plasmid Mini-prep kit (lot # 048k6062)as used. Standard manufacturer protocol used; elution in 50&#181;L of ddH<sub>2</sub>O.
Sigma Genelute Plasmid Mini-prep kit (lot # 048k6062)as used. Standard manufacturer protocol used; elution in 50&#181;L of ddH<sub>2</sub>O.
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<td width="100px">Plasmid sample</td>
<td width="100px">Plasmid sample</td>
<td width="50px">260/280</td>
<td width="50px">260/280</td>

Revision as of 21:12, 28 July 2009

University of Calgary

UNIVERSITY OF CALGARY



THIS MONTH

May
MTWTFSS
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25 26 27 28 29 30 31


NOTEBOOK PAGE INDEX
Carol
Chinmoyee
Emily
Fahd
Iman
Jamie
Jeremy
Katie
Kevin
Mandy
Patrick
Prima
Stefan
Vicki



CALENDAR

May
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May 26, 2009


CAROL

Descriptive Title of What You’re Doing

WIKI CODING HERE

CHINMOYEE

Descriptive Title of What You're Doing

WIKI CODING HERE


EMILY

Plasmid Isolation of LuxOD47E in pCR2.1 TOPO and psB1AC3 vectors

  • Protocol: Used Sigma GenElute Plasmid Miniprep Kit (Sigma), lot # 048k6062, used as according to the manufacturers instructions.



  • Results: Used Nanodrop 1000 Spectrophotometer read at 260 wavelength to determine DNA concentrations



Plasmid 260/280 260/230 Concentration [ng/μL]
LuxOD47E TOPO C1 1.88 2.21 409.5
LuxOD47E TOPO C2 1.86 2.18 551.4
LuxOD47E BBK C1 2.04 5.01 551.4
LuxOD47E BBK C2 1.77 1.39 92.9


FAHD

Descriptive Title of What You're Doing

WIKI CODING HERE


IMAN

Descriptive Title of What You're Doing

WIKI CODING HERE


JAMIE

Plasmid Isolation of luxOU in pCR-Blunt II-TOPO and psB1AC3

Sigma Genelute Plasmid Mini-prep kit (lot # 048k6062)as used. Standard manufacturer protocol used; elution in 50µL of ddH2O.

Plasmid sample 260/280 260/230 Concentration(ng/µL)
TOP10 luxOU C1 1.91 2.58 365.6
TOP10 luxOU C2 1.90 2.52 287.2
BBK luxOU C1 1.87 2.22 534.2
BBK luxOU C2 1.92 2.24 715.2

JEREMY

Descriptive Title of What You're Doing

WIKI CODING HERE


KATIE

Descriptive Title of What You're Doing

WIKI CODING HERE


KEVIN

Plasmid Isolation of Pqrr4 for Reporter circuit

The Pqrr4 was isolated from TOPO T/A and biobrick AC (pSB1AC3) using Sigma’s Genelute Plasmid Mini-prep kit (sigma) according to the specifications of the manufacturer. Nanodrop utility along with the Spectrophotometer was used to measure the concentration and purity of the isolated plasmid. The wavelength was set at 260nm.

Plasmid 260/280 260/230 Concentration [ng/μL]
Pqrr4 TOPO Blue tube
1.81
2.24
181
Pqrr4 TOPO Pink tube
1.81
2.02
231.3
Pqrr4 BBK Green tube
1.76
1.66
51.2
Pqrr4 BBK Pink tube 1.85 1.45 60.6
*The two TOPO vectors were from the same colony, thus labelled "Green tube" instead of C1. The two BBK plasmids were also from same colony, so same label applies.

Plasmid 260/280 260/230 Concentration [ng/μL]
Pqrr4 TOPO Blue Tube 1.81 2.24 181
Pqrr4 TOPO Pink Tube 1.81 2.02 231.3
Pqrr4 BBK Green Tube 1.76 1.66 51.2
Pqrr4 BBK Pink Tube 1.85 1.45 60.6


MANDY

Descriptive Title of What You're Doing

WIKI CODING HERE


PATRICK

Descriptive Title of What You're Doing

WIKI CODING HERE


PRIMA

Descriptive Title of What You're Doing

WIKI CODING HERE


STEFAN

Descriptive Title of What You're Doing

WIKI CODING HERE


VICKI

Descriptive Title of What You're Doing

WIKI CODING HERE

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