From 2009.igem.org
(Difference between revisions)
|
|
| Line 504: |
Line 504: |
| | <div class="desc"> | | <div class="desc"> |
| | </html> | | </html> |
| - | I have gotten some books from the library on scripting. I'm learning by using bits ans pieces from other people's free scripts and modifying them to do different | + | I have gotten some books from the library on scripting. I'm learning by using bits ans pieces from other people's free scripts and modifying them to do different things. I have managed to make a script that is adjustable to make an object move around randomly. |
| | | | |
| | <html> | | <html> |
Revision as of 17:28, 21 August 2009
University of Calgary
|
|
CAROL
Descriptive Title of What You’re Doing
WIKI CODING HERE
|
|
|
CHINMOYEE
Search for parameter values
Looked through many paper for estimates of initial values for each species in our model and estimates for the k values present in the model .
cell volume : 0.58 -0.69 um^3
Kubitschek HE. Cell volume increase in Escherichia coli after shifts to richer media. J Bacteriol. 1990 Jan172(1):94-101
RNA polymerase transcription rate :70nt/sec
Mutation per base pair per replication : Drake JW, A constant rate of spontaneous mutation in DNA-based microbes.Proc Natl Acad Sci U S A. 1991 Aug 1588(16):7160-4 Table 1
|
|
|
EMILY
Construction Begins!
Now that we have our gene of interest, LuxOD47E, Biobricked, we can start constuction to add the J13002 promoter and the B0015 terminator. We'll do two constrcutions in parallel and see if one of them works.
* Did a restriction digest cutting the insert with EcoRI and SpeI and cutting the recipient with EcoRI and XbaI restriction enzymes.
* Performed Antarctic Phosphotase Treatment (NEB) on the recipients, followed by ligation of the inserts and recipients.
*Transformed J13002-LuxOD47E into TOP10 cells and plated on AC and C plates in 25 uL and 50 uL, left plates for overnight growth.
*Did restreaks and made overnight cultures of colony2X LuxOD47E BBK.
|
|
|
FAHD
Descriptive Title of What You're Doing
|
|
|
IMAN
Descriptive Title of What You're Doing
|
|
|
JAMIE
Descriptive Title of What You're Doing
|
|
|
JEREMY
Descriptive Title of What You're Doing
|
|
|
KATIE
Descriptive Title of What You're Doing
|
|
|
KEVIN
1. Plasmid Isolation of fluorescent proteins
Isolation of fluorescent proteins were done in order to verify the presence and functionality of fluorescent proteins and promoters.
1. Testing of fluorescent proteins and the promoters
Isolated Flurorescent proteins were constructed behind of R0040 or J13002, depending on whether or not the fluorescent protein contained RBS, and transformed into TOP10 cells.
|
|
|
MANDY
Descriptive Title of What You're Doing
|
|
|
PATRICK
Descriptive Title of What You're Doing
|
|
|
PRIMA
Marketing
Today I spoke to the President from BioAlberta and he generously offered to donate money to iGEM Calgary. BioAlberta was added to our Bronze list for sponsorship.
I also wrote up some emails to companies after researching the companies for the past few days. The rest of the marketing team edited the emails before I sent them off. I also call 8 other companies and 5 companies were interested in looking at our sponsorship package. I sent off the package and updated the iGEM marketing excel spreadsheet. I left a voicemail to the 3 companies and I'll follow up with them tomorrow or later this week. I got the contact information of the Marketing Lead for one of those 3 companies. My plan is to call the other 2 companies tomorrow to first: get a hold of someone in-charge of sponsorship and second: speak to them about our project, potential applications and how it may benefit their company.
|
|
|
STEFAN
Scripting progress
I have gotten some books from the library on scripting. I'm learning by using bits ans pieces from other people's free scripts and modifying them to do different things. I have managed to make a script that is adjustable to make an object move around randomly.
|
|
|
VICKI
Descriptive Title of What You're Doing
|
"
