Team:Edinburgh/biology(materialsandmethods)

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Revision as of 21:44, 20 October 2009

Biology - Material and Methods
Protocol Name
Download Link
Comments
Creating Composite BioBrick
Transformation of Cells
Cloning BioBrick Parts by Fusion PRC
Protocol to Store Cell Cultures
Removal of PstI Site on onr Enzyme
Sequencing Protocol
Competent Cells Preparation
Transduction of E.Coli
Making of P1 Lysate
Quick E.coli transformations using CaCl2
PCR
Primers
Download Link
Comments
E.Coli Nitrate-Nitrite Sensing Upstream Primers
Enterobacter Cloacae Pentaerythritol Tetranitrate Reductase
Lux Operon Primers
Pentaerythritol Tetranitrate Reductase Primers
TNT.R1 A Receptor With Fair Affinity
TrzI Primers
Enterobacter Cloacae Pentaerythritol Tetranitrate Reductase (onr) Gene
Photobacterium Phosphoreum Lumazine Protein (lumP) Gene
Edinburgh University iGEM Team 2009

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