Team:Edinburgh/biology(materialsandmethods)

From 2009.igem.org

(Difference between revisions)

Revision as of 22:05, 20 October 2009

Overall Description & Design

TNT-Sensing Pathway

Nitrite/Nitrate-Sensing Pathway

Biobrick Parts

Results

Problem Solving and Tips

Materials and Methods

References

Biology - Material and Methods

Protocol Name	Download Link
Making Competent Cells	Download
Transforming E. coli cells by heat shock	Download
Transforming E. coli cells by CaC12	Download
Transducing of E. coli cells	Download
Making P1 lysate	Download
Storing Cell Cultures	Download
PCR using Kod Polymerase	Download
Fusion PCR	Download
Sequencing protocol	Download
Building Composite BioBrick Parts	Download

Primers	Download Link
E.Coli Nitrate-Nitrite Sensing Upstream Primers

Edinburgh University iGEM Team 2009

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 				<th><center>Primers</center></th>
 				<th><center>Download Link</center></th>
-                                <th><center>Comments</center></th>
 			</tr>
 			<tr>
 				<td><center><i>E.Coli</i> Nitrate-Nitrite Sensing Upstream Primers</center></td>
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-				<td><center>Enterobacter Cloacae Pentaerythritol Tetranitrate Reductase</center></td>
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-				<td><center>Lux Operon Primers</center></td>
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-			</tr>
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-				<td><center>Pentaerythritol Tetranitrate Reductase Primers</center></td>
-				<td><center></center></td>
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-			</tr>
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-				<td><center>TNT.R1 A Receptor With Fair Affinity</center></td>
-				<td><center></center></td>
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-			</tr>
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-				<td><center>TrzI Primers</center></td>
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-			</tr>
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-				<td><center>Enterobacter Cloacae Pentaerythritol Tetranitrate Reductase (onr) Gene</center></td>
-				<td><center></center></td>
-                                <td><center></center></td>
-			</tr>
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-				<td><center><i>Photobacterium Phosphoreum</i> Lumazine Protein (lumP) Gene</center></td>
-				<td><center></center></td>
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 			</tr>