Team:Edinburgh/biology(materialsandmethods)

From 2009.igem.org

Overall Description & Design

TNT-Sensing Pathway

Nitrite/Nitrate-Sensing Pathway

Biobrick Parts

Results

Problem Solving and Tips

Materials and Methods

References

• Home
• Biology
  • Overall Description and Design
  • TNT-Sensing Pathway
  • Nitrite/Nitrate-Sensing Pathway
  • Biobrick Parts
  • Results
  • Problem Solving and Tips
  • Materials and Methods
  • References
• Modelling
  • Overall Description
  • Gene Regulatory Network
  • Real Life Modelling
  • Scale Up
  • Results
  • References
• Underlying Philosophy
  • Public Perception
  • Legislation issues
  • Biosafety
  • DEMOCS Card Game
• Informatics
  • Introduction
  • Globe Tutorial
  • iGEM WIKI Hacks
  • Conclusions
• Notebook
• Team
  • Team Members
  • Advisors
  • Supervisors
  • Gallery
  • Contacts
  • Acknowledgements

Biology - Material and Methods

Protocol Name	Download Link	Comments
Creating Composite BioBrick
Transformation of Cells
Cloning BioBrick Parts by Fusion PRC
Protocol to Store Cell Cultures
Removal of PstI Site on onr Enzyme
Sequencing Protocol
Competent Cells Preparation
Transduction of E.Coli
Making of P1 Lysate
Quick E.coli transformations using CaCl2
PCR

Primers	Download Link	Comments
E.Coli Nitrate-Nitrite Sensing Upstream Primers
Enterobacter Cloacae Pentaerythritol Tetranitrate Reductase
Lux Operon Primers
Pentaerythritol Tetranitrate Reductase Primers
TNT.R1 A Receptor With Fair Affinity
TrzI Primers
Enterobacter Cloacae Pentaerythritol Tetranitrate Reductase (onr) Gene
Photobacterium Phosphoreum Lumazine Protein (lumP) Gene

Edinburgh University iGEM Team 2009

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