Team:Edinburgh/biology(materialsandmethods)

From 2009.igem.org

Overall Description & Design

TNT-Sensing Pathway

Nitrite/Nitrate-Sensing Pathway

Biobrick Parts

Results

Problem Solving and Tips

Materials and Methods

References

• Home
• Biology
  • Overall Description and Design
  • TNT-Sensing Pathway
  • Nitrite/Nitrate-Sensing Pathway
  • Biobrick Parts
  • Results
  • Problem Solving and Tips
  • Materials and Methods
  • References
• Modelling
  • Overall Description
  • Gene Regulatory Network
  • Real Life Modelling
  • Scale Up
  • Results
  • References
• Underlying Philosophy
  • Public Perception
  • Legislation issues
  • Biosafety
  • DEMOCS Card Game
• Informatics
  • Introduction
  • Globe Tutorial
  • iGEM WIKI Hacks
  • Conclusions
• Notebook
• Team
  • Team Members
  • Advisors
  • Supervisors
  • Gallery
  • Contacts
  • Acknowledgements

Biology - Material and Methods

Protocol Name	Download Link
Making Competent Cells	Download

Primers	Download Link	Comments
E.Coli Nitrate-Nitrite Sensing Upstream Primers
Enterobacter Cloacae Pentaerythritol Tetranitrate Reductase
Lux Operon Primers
Pentaerythritol Tetranitrate Reductase Primers
TNT.R1 A Receptor With Fair Affinity
TrzI Primers
Enterobacter Cloacae Pentaerythritol Tetranitrate Reductase (onr) Gene
Photobacterium Phosphoreum Lumazine Protein (lumP) Gene

Edinburgh University iGEM Team 2009

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