Team:Edinburgh/biology(materialsandmethods)

From 2009.igem.org

Overall Description & Design

TNT-Sensing Pathway

Nitrite/Nitrate-Sensing Pathway

Biobrick Parts

Results

Problem Solving and Tips

Materials and Methods

References

• Home
• Biology
  • Overall Description and Design
  • TNT-Sensing Pathway
  • Nitrite/Nitrate-Sensing Pathway
  • Biobrick Parts
  • Results
  • Problem Solving and Tips
  • Materials and Methods
  • References
• Modelling
  • Overall Description
  • Gene Regulatory Network
  • Real Life Modelling
  • Scale Up
  • Results
  • References
• Underlying Philosophy
  • Public Perception
  • Legislation issues
  • Biosafety
  • DEMOCS Card Game
• Informatics
  • Introduction
  • Globe Tutorial
  • iGEM WIKI Hacks
  • Conclusions
• Notebook
• Team
  • Team Members
  • Advisors
  • Supervisors
  • Gallery
  • Contacts
  • Acknowledgements

Biology - Material and Methods

Protocol Name	Download Link
Making Competent Cells	Download
Transforming E. coli cells by heat shock	Download
Transforming E. coli cells by CaC12	Download
Transducing of E. coli cells	Download
Making P1 lysate	Download
Storing Cell Cultures	Download
PCR using Kod Polymerase	Download
Fusion PCR	Download
Sequencing protocol	Download
Building Composite BioBrick Parts	Download

Primers	Download Link	Comments
E.Coli Nitrate-Nitrite Sensing Upstream Primers
Enterobacter Cloacae Pentaerythritol Tetranitrate Reductase
Lux Operon Primers
Pentaerythritol Tetranitrate Reductase Primers
TNT.R1 A Receptor With Fair Affinity
TrzI Primers
Enterobacter Cloacae Pentaerythritol Tetranitrate Reductase (onr) Gene
Photobacterium Phosphoreum Lumazine Protein (lumP) Gene

Edinburgh University iGEM Team 2009

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