Team:Kyoto/GSDD/Notebook/0817-0823

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==0810-0816 : what to do. what to do. what to do. what to do. ==
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==0817-0823 : Construction.PCR.==
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===Tuesday, 18 August===
===Tuesday, 18 August===
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We use pSB1A2 in order to insert PCR products and sequence them. But the concentrations of this sample are very low. So, we will try again.
We use pSB1A2 in order to insert PCR products and sequence them. But the concentrations of this sample are very low. So, we will try again.
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===Friday, 21 August===
===Friday, 21 August===

Latest revision as of 02:20, 22 October 2009

  1. Home
  2. GSDD
  3. Notebook

0727-0802
Construction.
0803-0809
Construction.
0810-0816
Construction.
0817-0823
Construction.PCR.
0824-0830
Construction.PCR.
0831-0906
Construction.PCR.MPR.
0907-0913
Construction.PCR.MPR.
0914-0920
Construction.PCR.MPR.
0921-0927
Construction.PCR.MPR.
0928-1004
Construction.PCR.MPR.
1005-1011
Construction.PCR.MPR.
1012-1018
Construction.MPR.Observation.

0817-0823 : Construction.PCR.


Tuesday, 18 August

To Do

  • PCR
  • Restriction Enzyme Digestion
  • Electrophoresis
  • Gel Extraction kit
    • 3-O-2
    • pSB1A2

Results

3-O-2
primerF
AACTGCAGAACCAATGCATTGGagaacgtctctcactgaac
primerR
aaaattttGGACTAGTCCttgaatcttagcccccttg

Kyoto 0818 1.jpg

  • Length
3-O-2+BBa_J63010
6103bp
3-O-2 PCR product
2851bp

We can not find the bands of PCR products. So, we will do PCR again, changing the volume of MgSO4, 3ul, 4.5ul, 6ul.

pSB1A2

Kyoto 0818 2.jpg

  • Length
pSB1A2
2079bp
conc.
sample nameconc./(ng/ul)
pSB1A2(EcoR1,Spe1)9.5
pSB1A2(Xba1,Pst1)5.6

We use pSB1A2 in order to insert PCR products and sequence them. But the concentrations of this sample are very low. So, we will try again.



Friday, 21 August

To Do

  • PCR
  • Restriction Enzyme Digestion
  • Electrophoresis
  • Gel Extraction kit
    • 3-O-2
    • pSB1A2


Results

3-O-2
primerF
AACTGCAGAACCAATGCATTGGagaacgtctctcactgaac
primerR
aaaattttGGACTAGTCCttgaatcttagcccccttg
  • Length
3-O-2+BBa_J63010
6103bp
3-O-2 PCR product
2851bp
pSB1A2
  • Length
pSB1A2
2079bp
conc.
sample nameconc./(ng/ul)
pSB1A2(EcoR1,Spe1)13.4
pSB1A2(Xba1,Pst1)11.9

We use pSB1A2 in order to insert PCR products and sequence them.