Team:Kyoto/GSDD/Notebook/0831-0904

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(Difference between revisions)
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=====Miniprep=====
=====Miniprep=====
*p2-1-1, p2-1-2, p2-4-2 was minipreped.
*p2-1-1, p2-1-2, p2-4-2 was minipreped.
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{|
+
{| class="table"
|+Miniprep conc.
|+Miniprep conc.
!NO.!!Sample name!!conc./ul
!NO.!!Sample name!!conc./ul
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=====MPR=====
=====MPR=====
*xxx was MPRed.
*xxx was MPRed.
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{|
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|+18 MPR samples
|+18 MPR samples
!NO.!!1!!2!!3!!4!!5!!6
!NO.!!1!!2!!3!!4!!5!!6

Revision as of 07:37, 3 October 2009

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0727-0802
Construction.
0803-0809
Construction.
0810-0816
Construction.
0817-0823
Construction.PCR.
0824-0830
Construction.PCR.
0831-0906
Construction.PCR.MPR.
0907-0913
Construction.PCR.MPR.
0914-0920
Construction.PCR.MPR.
0921-0927
Construction.PCR.MPR.
0928-1004
Construction.PCR.MPR.
1005-1011
Construction.PCR.MPR.
1012-1018
Construction.MPR.Observation.

Week 1 : what to do. what to do. what to do. what to do.

Monday, 31 August

To Do

  • Miniprep p2-1-1, p2-1-2, p2-4-2
  • Transformation p2-4-1(retry)
  • MPR

Results

Miniprep
  • p2-1-1, p2-1-2, p2-4-2 was minipreped.
Miniprep conc.
NO.Sample nameconc./ul
1p2-1-167.9
2p2-1-261.5
3p2-4-294.4
Transformation
  • p2-4-1 was transformed, and colony was observed.
MPR
  • xxx was MPRed.
18 MPR samples
NO.123456
temp.X deg C606060606060
MPR primer 1Fw1Fw2Fw3Fw1Fw2Fw3
MPR primer 2Rev1Rev1Rev1Rev2Rev2Rev2
NO.123456
temp.X deg C636363636363
MPR primer 1Fw1Fw2Fw3Fw1Fw2Fw3
MPR primer 2Rev1Rev1Rev1Rev2Rev2Rev2
NO.123456
temp.X deg C666666666666
MPR primer 1Fw1Fw2Fw3Fw1Fw2Fw3
MPR primer 2Rev1Rev1Rev1Rev2Rev2Rev2

Kyoto MPR 0831.png

  • Bands were observed on samples with Fw2.
  • Bands were longer on samples with Rev1 than with Rev2.

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