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First Stage Assembly
Design
Several strategies are designed for the first stage assembly, the assembly includes three different parts that we constructed. One of them is the AND Gate Module (without output), the second is the Bistable module that we inherited from iGEM 2007 Team Peking, and the third part T7promoter CI acts as a bridge which is both the output of the AND Gate and the input to the bistable module.
Considering the size of the plasmid and replication origin compatibility of the plasmids, we draw up the following methods to assembly the system by making combinations among the available plasmids and those parts. What we wanted to do is to pick out a result that most approaches our need. And then further carry out mutation on the ribosome binding site for fine tuning if necessary.
Second Stage Assembly (In Process)
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