Team:PKU Beijing/Project/Output
From 2009.igem.org
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In [http://parts.mit.edu/igem07/index.php/Cambridge/Amplifier_project Cambridge 2007 Amplifier project], the PhiR73 activator and the PO promoter is used to amplify signal from an inducible promoter. | In [http://parts.mit.edu/igem07/index.php/Cambridge/Amplifier_project Cambridge 2007 Amplifier project], the PhiR73 activator and the PO promoter is used to amplify signal from an inducible promoter. | ||
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+ | [[Image:PKU_Cambridge_AgrAmp.png|400px|thumb|center|fig2. Cambridge 2007 amplifier project ''picture from [http://parts.mit.edu/igem07/index.php/Cambridge/Amplifier_project Cambridge 2007 page'']]] | ||
In order to make the transcription level from PO promoter similar to that of the sal promoter, the activator is designed to be on a lower copy plasmid than the reporter. This is also discussed in the second stage assembly section. | In order to make the transcription level from PO promoter similar to that of the sal promoter, the activator is designed to be on a lower copy plasmid than the reporter. This is also discussed in the second stage assembly section. |
Revision as of 06:50, 15 October 2009