Team:PKU Beijing/Project/Output
From 2009.igem.org
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{{PKU_Beijing/Sidebar_Project}} | {{PKU_Beijing/Sidebar_Project}} | ||
{{PKU_Beijing/Header2}} | {{PKU_Beijing/Header2}} | ||
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+ | ==='''Design'''=== | ||
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+ | The OR gate and output module is constructed as below: | ||
+ | Salicylate promter is placed upstream of the PO promoter, the double promoter construct can act as an OR Gate, either PhiR73 or salicylate can activate the promoter. | ||
[[Image:PKU_OR_OUTPUT.png|600px|thumb|center|fig1. OR Gate and Output]] | [[Image:PKU_OR_OUTPUT.png|600px|thumb|center|fig1. OR Gate and Output]] | ||
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+ | In [http://parts.mit.edu/igem07/index.php/Cambridge/Amplifier_project Cambridge 2007 Amplifier project], the PhiR73 activator and the PO promoter is used to amplify signal from an inducible promoter. | ||
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+ | In order to make the transcription level from PO promoter similar to that of the sal promoter, the activator is designed to be on a lower copy plasmid than the reporter. This is also discussed in the second stage assembly section. | ||
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+ | ==='''Testing'''=== | ||
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+ | In order to test whether the OR Gate works, we constructed the following 2 strains of ''E.coli'' to test each of the two promoters. | ||
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{{PKU_Beijing/Foot}} | {{PKU_Beijing/Foot}} |
Revision as of 06:47, 15 October 2009
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