Team:Paris/3 August 2009
From 2009.igem.org
(Difference between revisions)
(→Lab work) |
(→Lab work) |
||
Line 37: | Line 37: | ||
Miniprep digestion | Miniprep digestion | ||
</div><div style="background-color:#E0E9EF; border:1px solid #333333; padding:3px 5px; margin-bottom:10px"> | </div><div style="background-color:#E0E9EF; border:1px solid #333333; padding:3px 5px; margin-bottom:10px"> | ||
- | *For [[https://2009.igem.org/Team:Paris/Freezer#Plasmids:_MiniPrep_Products P1]] pSB2K3, [[https://2009.igem.org/Team:Paris/Freezer#Plasmids:_MiniPrep_Products P2]] BBa_J61002, [[https://2009.igem.org/Team:Paris/Freezer#Plasmids:_MiniPrep_Products P3]] BBa_J32015, [[https://2009.igem.org/Team:Paris/Freezer#Plasmids:_MiniPrep_Products P4]] | + | *For [[https://2009.igem.org/Team:Paris/Freezer#Plasmids:_MiniPrep_Products P1]] pSB2K3, [[https://2009.igem.org/Team:Paris/Freezer#Plasmids:_MiniPrep_Products P2]] BBa_J61002, [[https://2009.igem.org/Team:Paris/Freezer#Plasmids:_MiniPrep_Products P3]] BBa_J32015, [[https://2009.igem.org/Team:Paris/Freezer#Plasmids:_MiniPrep_Products P4]] BBa_B0014, [[https://2009.igem.org/Team:Paris/Freezer#Plasmids:_MiniPrep_Products P5]] BBa_I14032, [[https://2009.igem.org/Team:Paris/Freezer#Plasmids:_MiniPrep_Products P7]] BBa_R0040, [[https://2009.igem.org/Team:Paris/Freezer#Plasmids:_MiniPrep_Products P8]] BBa_I0500. |
*Digestion Mix | *Digestion Mix | ||
**50µl Miniprep | **50µl Miniprep |
Revision as of 07:56, 4 August 2009
Contents |
NoteBook
|
|
|
|
|
---|
Brain work
Meeting this morning for the lab work week. Discuss about the schedule algorithm for the iPhone application.
Lab work
DID dying for membrane fluorescence microscopy
- No result = we have to try another method
Miniprep digestion
Gel Migration
Culture ON for Miniprep
- [S7] DH5α(g3p)
To do list
Matricule | TODO |
Luc | SNARE Construction |
Romain | Design oligo Fec A |
Charlotte | design of oligos: FecR, FecA, SNARE?, sequencing oligo |
Stoff | Dev schedule algorithm |
Chris | modelling: Tol-Pal system, delay system, first equations in biocham ; use of mathlab. Think about the transduction system |
Lisa | Oligo Tol/Pal / Oligo Sequencing / Tol/Pal Protocol verification / Keio / Theme A writting |
Caroline | coloration of cells / protocols for the lab: control of the emission of vesicules.... |
Souf | design of oligos: FecR, FecA, SNARE?, sequencing oligo |
Vicard | planification of all the steps necessary for the implementation of the ClyA device in our strains / search for articles on fusion of vesicules |
Pierre | modelling: Tol-Pal system, delay system, first equations in biocham ; use of mathlab. Think about the transduction system |
Sylvain | minipreps. Check if the use of starch is possible ; problem : find a system to help the starch enter into the periplasm, use of LacY -> noise |
Guillaume | miniprep digestion using Xba/Pst enzymes |