Team:Paris/Addressing testing
From 2009.igem.org
(→Adressing system) |
(→Adressing system) |
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- | '''global | + | '''global constructions :''' |
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+ | Time required : | ||
+ | |||
+ | Experiments ran : | ||
+ | |||
+ | PCR : on plasmid ? Oligo TM | ||
+ | |||
+ | 2 | ||
+ | |||
+ | Verification on gel | ||
+ | |||
+ | Purification on gel | ||
+ | |||
+ | digestion: | ||
+ | |||
+ | ligation: | ||
+ | |||
+ | PCR colo : | ||
+ | |||
+ | miniprep: | ||
+ | |||
+ | verifivation of the miniprep by digestion | ||
+ | |||
+ | sequencing | ||
[['''ClyA''']] | [['''ClyA''']] | ||
Revision as of 11:09, 17 October 2009
iGEM > Paris > WetLab > Addressing
Adressing system
global constructions :
Time required :
Experiments ran :
PCR : on plasmid ? Oligo TM
2
Verification on gel
Purification on gel
digestion:
ligation:
PCR colo :
miniprep:
verifivation of the miniprep by digestion
sequencing [[ClyA]]
-ClyA Nterm= biobrick+sequencage+stock glycerol Done
-ClyA Cterm= biobrick+sequencage+stock glycerol Done
[[-RFP-]]
-RFP Nterm= biobrick+sequencage+stock glycerol Done
-RFP Cterm= biobrick+sequencage+stock glycerol Done
[[-ClyA Cter-RFP Nter-]]
-ClyA Cter-RFP Nter= biobrick+stock glycerol Done
[[-RFP Cter-ClyA Nter-]]
-miss ligation between 2 parts (j'ai fait plusieurs ligation le 7ou8/09, mais pas marché le 9/09 apres culture overnight, voir si les autres ont continué)
[[PBad-ClyA Cter-RFP Nter-]]
-failed many time with:
Pbad cut in S/P and ClyA Cter-RFP Nter in X/P
Pbad cut in E/S and ClyA Cter-RFP Nter in E/X