Team:Paris/Addressing testing
From 2009.igem.org
(→Adressing system) |
(→Adressing system) |
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- | Time required : | + | Time required : A lOT !!!!! |
Experiments ran : | Experiments ran : | ||
- | + | From plate: mRFP | |
- | + | PCR : | |
+ | |||
+ | ClyA Cterm plasmid : ? Oligo : TM : | ||
+ | ClyA Nterm plasmid : ? Oligo : TM : | ||
+ | mRFP Cterm plasmid : ? Oligo : TM : | ||
+ | mRFP Nterm plasmid : ? Oligo : TM : | ||
Verification on gel | Verification on gel | ||
+ | ok | ||
+ | |||
Purification on gel | Purification on gel | ||
+ | |||
+ | ok | ||
digestion: | digestion: | ||
+ | |||
+ | ok | ||
ligation: | ligation: | ||
+ | |||
+ | |||
PCR colo : | PCR colo : |
Revision as of 11:13, 17 October 2009
iGEM > Paris > WetLab > Addressing
Adressing system
global constructions :
Time required : A lOT !!!!!
Experiments ran :
From plate: mRFP
PCR :
ClyA Cterm plasmid : ? Oligo : TM : ClyA Nterm plasmid : ? Oligo : TM : mRFP Cterm plasmid : ? Oligo : TM : mRFP Nterm plasmid : ? Oligo : TM :
Verification on gel
ok
Purification on gel
ok
digestion:
ok
ligation:
PCR colo :
miniprep:
verifivation of the miniprep by digestion
sequencing [[ClyA]]
-ClyA Nterm= biobrick+sequencage+stock glycerol Done
-ClyA Cterm= biobrick+sequencage+stock glycerol Done
[[-RFP-]]
-RFP Nterm= biobrick+sequencage+stock glycerol Done
-RFP Cterm= biobrick+sequencage+stock glycerol Done
[[-ClyA Cter-RFP Nter-]]
-ClyA Cter-RFP Nter= biobrick+stock glycerol Done
[[-RFP Cter-ClyA Nter-]]
-miss ligation between 2 parts (j'ai fait plusieurs ligation le 7ou8/09, mais pas marché le 9/09 apres culture overnight, voir si les autres ont continué)
[[PBad-ClyA Cter-RFP Nter-]]
-failed many time with:
Pbad cut in S/P and ClyA Cter-RFP Nter in X/P
Pbad cut in E/S and ClyA Cter-RFP Nter in E/X