Team:Paris/Addressing testing

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WetLab

Main - Addressing - Production - reception - Addressing

Adressing system

global constructions :

Time required : A lOT !!!!!

Experiments ran :

Date	Authors	Article
[]	1999	Terry J. Beveridge	Structures of gram-negative cell walls and their derived membrane vesicles.	10438737
[]	2003	Miller SI & Guina T.	Bacterial vesicle formation as a mechanism of protein transfer to animals.	14531993
[]	2005	Kuehn MJ & Kesty NC.	Bacterial outer membrane vesicles and the host-pathogen interaction.	16291643
[]	2006	McBroom AJ & Kuehn MJ.	Outer membrane vesicle production by Escherichia coli is independent of membrane instability.	16855227
[]	2007	McBroom AJ & Kuehn MJ.	Release of outer membrane vesicles by Gram-negative bacteria is a novel envelope stress response.	17163978
[]	2009	Deatherage BL & Cookson BT.	Biogenesis of bacterial membrane vesicles	19432795
[]	2009	Purnick PE & Weiss R.	The second wave of synthetic biology: from modules to systems.	19461664

From plate: mRFP

PCR :

ClyA Cterm plasmid : ? Oligo : TM : ClyA Nterm plasmid : ? Oligo : TM : mRFP Cterm plasmid : ? Oligo : TM : mRFP Nterm plasmid : ? Oligo : TM :

Verification on gel

ok

Purification on gel

ok

digestion:

ClyA Cterm S/P RFP Nterm X/P PBAD E/S

ligation:

ClyA Cter-RFP Nter vector PSB1A3

PCR colo :

miniprep:

verifivation of the miniprep by digestion

sequencing [[ClyA]]

-ClyA Nterm= biobrick+sequencage+stock glycerol Done

-ClyA Cterm= biobrick+sequencage+stock glycerol Done

[[-RFP-]]

-RFP Nterm= biobrick+sequencage+stock glycerol Done

-RFP Cterm= biobrick+sequencage+stock glycerol Done

[[-ClyA Cter-RFP Nter-]]

-ClyA Cter-RFP Nter= biobrick+stock glycerol Done

[[-RFP Cter-ClyA Nter-]]

-miss ligation between 2 parts (j'ai fait plusieurs ligation le 7ou8/09, mais pas marché le 9/09 apres culture overnight, voir si les autres ont continué)

[[PBad-ClyA Cter-RFP Nter-]]

-failed many time with:

Pbad cut in S/P and ClyA Cter-RFP Nter in X/P

Pbad cut in E/S and ClyA Cter-RFP Nter in E/X

Export system

@@ Line 25: / Line 25: @@
 Time required : A lOT !!!!!
 Experiments ran :
+{|
+|- style="background: #0a3585; text-align: center; color:white; font-weight:bold; "
+|width= 300px| Date
+|width=300px| Authors
+|width=300px| Article
+|- style="background: #bebebe; text-align: center;"
+|height=40px| []
+|1999
+|Terry J. Beveridge
+|Structures of gram-negative cell walls and their derived membrane vesicles.
+|[http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=93954 10438737]
+|- style="background: #d8d8d8; text-align: center;"
+|height=40px|[]
+|2003
+|Miller SI & Guina T.
+|Bacterial vesicle formation as a mechanism of protein transfer to animals.
+|[http://www.ncbi.nlm.nih.gov/pubmed/14531993?ordinalpos=&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.SmartSearch&log$=citationsensor 14531993]
+|- style="background: #bebebe; text-align: center;"
+|height=40px|[]
+|2005
+|Kuehn MJ & Kesty NC.
+|Bacterial outer membrane vesicles and the host-pathogen interaction.
+|[http://www.ncbi.nlm.nih.gov/pubmed/16291643?ordinalpos=&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.SmartSearch&log$=citationsensor 16291643]
+|- style="background: #d8d8d8; text-align: center;"
+|height=40px|[]
+|2006
+|McBroom AJ & Kuehn MJ.
+|Outer membrane vesicle production by Escherichia coli is independent of membrane instability.
+|[http://www.ncbi.nlm.nih.gov/pubmed/16855227?ordinalpos=&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.SmartSearch&log$=citationsensor 16855227]
+|- style="background: #bebebe; text-align: center;"
+|height=40px|[]
+|2007
+|McBroom AJ & Kuehn MJ.
+|Release of outer membrane vesicles by Gram-negative bacteria is a novel envelope stress response.
+|[http://www.ncbi.nlm.nih.gov/pubmed/17163978?ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum 17163978]
+|- style="background: #d8d8d8; text-align: center;"
+|height=40px|[]
+|2009
+|Deatherage BL & Cookson BT.
+|Biogenesis of bacterial membrane vesicles
+|[http://www.ncbi.nlm.nih.gov/pubmed/19432795?ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum 19432795]
+|- style="background: #bebebe; text-align: center;"
+|height=40px|[]
+|2009
+|Purnick PE & Weiss R.
+|The second wave of synthetic biology: from modules to systems.
+|[http://www.ncbi.nlm.nih.gov/pubmed/19461664?ordinalpos=&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.SmartSearch&log$=citationsensor 19461664]
+|}
 From plate: mRFP