Team:Paris/Addressing testing
From 2009.igem.org
(→Adressing system) |
(→Adressing system) |
||
Line 47: | Line 47: | ||
mRFP Nterm plasmid : ? Oligo : TM : | mRFP Nterm plasmid : ? Oligo : TM : | ||
+ | |||
|'''PCR :''' | |'''PCR :''' | ||
Line 66: | Line 67: | ||
|- style="background: #bebebe; text-align: center;" | |- style="background: #bebebe; text-align: center;" | ||
- | |Purification on gel : | + | |'''Purification on gel :''' |
- | |Purification on gel : | + | |
- | |Purification on gel : | + | ok |
+ | |||
+ | |'''Purification on gel :''' | ||
+ | |||
+ | ok | ||
+ | |||
+ | |'''Purification on gel :''' | ||
+ | |||
+ | ok | ||
+ | |||
|- style="background: #d8d8d8; text-align: center;" | |- style="background: #d8d8d8; text-align: center;" |
Revision as of 11:40, 17 October 2009
iGEM > Paris > WetLab > Addressing
Adressing system
global constructions :
Time required : A lOT !!!!!
Experiments ran :
row 1 | row 2 | row 3 |
PCR :
ClyA Cterm plasmid : ? Oligo : TM : ClyA Nterm plasmid : ? Oligo : TM : mRFP Cterm plasmid : ? Oligo : TM : mRFP Nterm plasmid : ? Oligo : TM :
| PCR : | PCR :
|
Verification on gel :
ok | Verification on gel :
ok | Verification on gel :
ok |
Purification on gel :
ok | Purification on gel :
ok | Purification on gel :
ok
|
digestion: | digestion: | digestion: |
verification digestion: | verification digestion: | verification digestion:
|
ligation: | ligation: | ligation:
|
PCR colo : | PCR colo : | PCR colo : |
miniprep: | miniprep: | miniprep:
|
sequencing : | sequencing : | sequencing : |
From plate: mRFP
PCR :
Verification on gel
ok
Purification on gel
ok
digestion:
ClyA Cterm S/P RFP Nterm X/P PBAD E/S
verification digestion:
ligation:
ClyA Cter-RFP Nter vector PSB1A3
PCR colo :
miniprep:
verifivation of the miniprep by digestion
sequencing [[ClyA]]
-ClyA Nterm= biobrick+sequencage+stock glycerol Done
-ClyA Cterm= biobrick+sequencage+stock glycerol Done
[[-RFP-]]
-RFP Nterm= biobrick+sequencage+stock glycerol Done
-RFP Cterm= biobrick+sequencage+stock glycerol Done
[[-ClyA Cter-RFP Nter-]]
-ClyA Cter-RFP Nter= biobrick+stock glycerol Done
[[-RFP Cter-ClyA Nter-]]
-miss ligation between 2 parts (j'ai fait plusieurs ligation le 7ou8/09, mais pas marché le 9/09 apres culture overnight, voir si les autres ont continué)
[[PBad-ClyA Cter-RFP Nter-]]
-failed many time with:
Pbad cut in S/P and ClyA Cter-RFP Nter in X/P
Pbad cut in E/S and ClyA Cter-RFP Nter in E/X