Team:Paris/Addressing testing

(Difference between revisions)

Revision as of 12:13, 17 October 2009

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global constructions :

Time required : A lOT !!!!!

Experiments ran :

row 1	row 2	row 3
PCR : ClyA Cterm matrix : ? Oligo : TM : ClyA Nterm matrix : ? Oligo : TM : mRFP Cterm matrix : ? Oligo : TM : mRFP Nterm plasmid : ? Oligo : TM :	PCR : -	PCR : -
Verification on gel : ok	Verification on gel : -	Verification on gel : -
Purification on gel : ok	Purification on gel : -	Purification on gel : -
digestion: ClyA Cterm S/P RFP Nterm X/P PBAD vector : ? E/S	digestion: ClyA Cter-RFP Nter vector PSB1A3 E/X	digestion: -
verification digestion: ok	verification digestion: ok	verification digestion: -
ligation: ClyA Cter-RFP Nter vector PSB1A3	ligation: PBAD E/S ClyA Cter-RFP Nter vector PSB1A3 E/X (x2) PBAD S/P ClyA Cter-RFP Nter vector PS X/P (x2)	ligation:
PCR colo : ok	PCR colo : ok	PCR colo : -
miniprep: clone :	miniprep: clone :	miniprep: -
sequencing : ok	sequencing : ok	sequencing : -
stock glycerol: ok	stock glycerol ok	stock glycerol -

Pbad cut in S/P and ClyA Cter-RFP Nter in X/P

Pbad cut in E/S and ClyA Cter-RFP Nter in E/X

Time required : A lOT !!!!!

Experiments ran :

@@ Line 123: / Line 123: @@
 |'''ligation:'''
-PBAD  vector : ? E/S  ClyA Cter-RFP Nter vector PSB1A3E/X (x2)
+PBAD  E/S  ClyA Cter-RFP Nter vector PSB1A3 E/X (x2)
-PBAD  vector : ? S/P  ClyA Cter-RFP Nter X/P (x2)
+PBAD  S/P  ClyA Cter-RFP Nter vector PS  X/P (x2)
 |'''ligation:'''