Team:Paris/Addressing testing

From 2009.igem.org

(Difference between revisions)
(→Adressing system)
(→Export system)
Line 207: Line 207:
-
Time required : A lOT !!!!!
+
Time required : A week !
Line 223: Line 223:
|'''PCR :'''  
|'''PCR :'''  
-
 
+
TatABCE matrix :
-
 
+
|'''PCR :'''
|'''PCR :'''
Line 237: Line 236:
|'''Verification on gel :'''
|'''Verification on gel :'''
-
ok
+
-
|'''Verification on gel :'''
|'''Verification on gel :'''
-
ok
+
-
|- style="background: #bebebe; text-align: center;"   
|- style="background: #bebebe; text-align: center;"   
Line 250: Line 249:
|'''Purification on gel :'''
|'''Purification on gel :'''
-
ok
+
-
|'''Purification on gel :'''
|'''Purification on gel :'''
-
ok
+
-
Line 260: Line 259:
|'''digestion:'''  
|'''digestion:'''  
-
 
+
TatABCE
-
 
+
-
 
+
|'''digestion:'''  
|'''digestion:'''  
Line 274: Line 271:
|'''verification digestion:'''
|'''verification digestion:'''
-
ok
+
-
|'''verification digestion:'''
|'''verification digestion:'''
-
ok
+
-
Line 284: Line 281:
|'''ligation:'''  
|'''ligation:'''  
-
 
+
TatABCE
|'''ligation:'''  
|'''ligation:'''  
Line 297: Line 294:
|'''PCR colo :'''
|'''PCR colo :'''
-
ok
+
-
|'''PCR colo :'''
|'''PCR colo :'''
-
ok
+
-
-
 
+
|- style="background: #d8d8d8; text-align: center;"
|- style="background: #d8d8d8; text-align: center;"
|'''miniprep:'''
|'''miniprep:'''
-
clone :
+
STOPPED
|'''miniprep:'''
|'''miniprep:'''
-
clone :
+
-
|'''miniprep:'''
|'''miniprep:'''
-
clone :
+
-
-
 
+
|- style="background: #bebebe; text-align: center;"   
|- style="background: #bebebe; text-align: center;"   
|'''sequencing :'''
|'''sequencing :'''
-
ok
+
-
|'''sequencing :'''
|'''sequencing :'''
-
ok
+
-
|'''sequencing :'''
|'''sequencing :'''
-
ok
+
-
|}
|}

Revision as of 12:21, 17 October 2009

iGEM > Paris > WetLab > Addressing

WetLab

Main - Addressing - Production - reception -
Addressing


Adressing system

global constructions :


plasmid = PSB3T5


Time required : A lOT !!!!!



Experiments ran :

row 1 row 2 row 3
PCR :

ClyA Cterm matrix : ? Oligo : TM :

ClyA Nterm matrix : ? Oligo : TM :

mRFP Cterm matrix : ? Oligo : TM :

mRFP Nterm plasmid : ? Oligo : TM :


PCR :

-

PCR :

-

Verification on gel :

ok

Verification on gel :

-

Verification on gel :

-

Purification on gel :

ok

Purification on gel :

-

Purification on gel :

-


digestion:

ClyA Cterm S/P

RFP Nterm X/P

PBAD vector : ? E/S


digestion:

ClyA Cter-RFP Nter vector PSB1A3 E/X

digestion:

-

verification digestion:

ok

verification digestion:

ok

verification digestion:

-


ligation:

ClyA Cter-RFP Nter vector PSB1A3

ligation:

PBAD E/S ClyA Cter-RFP Nter PSB1A3 E/X (x2) PBAD S/P ClyA Cter-RFP Nter PSB? X/P (x2)

ligation:


PCR colo :

ok

PCR colo :

ok

PCR colo :

-


miniprep:

clone :

miniprep:

clone :

miniprep:

-


sequencing :

ok

sequencing :

ok

sequencing :

-

stock glycerol:

S? S?

stock glycerol

S?

stock glycerol

-



Export system

Production of protein of interest



Production of protein of interest


Time required : A week !



Experiments ran :

row 1 row 2 row 3
PCR :

TatABCE matrix :

PCR : PCR :


Verification on gel :

ok

Verification on gel :

-

Verification on gel :

-

Purification on gel :

ok

Purification on gel :

-

Purification on gel :

-


digestion:

TatABCE

digestion: digestion:
verification digestion:

ok

verification digestion:

-

verification digestion:

-


ligation:

TatABCE

ligation: ligation:


PCR colo :

ok

PCR colo :

-

PCR colo :

-

miniprep:

STOPPED

miniprep:

-

miniprep:

-

sequencing :

-

sequencing :

-

sequencing :

-

Retrieved from "http://2009.igem.org/Team:Paris/Addressing_testing"