Team:Paris/Addressing testing

From 2009.igem.org

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|- style="background: #bebebe; text-align: center;"
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|'''Purification on gel :'''
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ClyA Cter-RFP Nter vector PSB1A3 E/X
ClyA Cter-RFP Nter vector PSB1A3 E/X
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|'''digestion:'''
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|'''digestion:'''  
|'''digestion:'''  
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ok
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|'''verification digestion:'''
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|'''verification digestion:'''
|'''verification digestion:'''
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PBAD  S/P  ClyA Cter-RFP Nter PSB?  X/P (x2)
PBAD  S/P  ClyA Cter-RFP Nter PSB?  X/P (x2)
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|'''ligation:'''  
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|'''ligation:'''
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|'''ligation:'''
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|'''PCR colo :'''
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|'''PCR colo :'''
|'''PCR colo :'''
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clone :
clone :
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|'''miniprep:'''
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|'''miniprep:'''
|'''miniprep:'''
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|'''sequencing :'''
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|'''sequencing :'''
|'''sequencing :'''
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S?
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|'''stock glycerol'''
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|'''stock glycerol'''
|'''stock glycerol'''

Revision as of 09:49, 18 October 2009

iGEM > Paris > WetLab > Addressing

WetLab

Main - Addressing - Production - reception -
Addressing


Adressing system

global constructions :


plasmid = PSB3T5


Time required : A lOT !!!!!



Experiments ran :

row 1 row 2 row 3 row 4
PCR :

ClyA Cterm matrix : ? Oligo : TM :

ClyA Nterm matrix : ? Oligo : TM :

mRFP Cterm matrix : ? Oligo : TM :

mRFP Nterm plasmid : ? Oligo : TM :


PCR :

-

PCR :

-

PCR :

-


Verification on gel :

ok

Verification on gel :

-

Verification on gel :

-

Purification on gel :

ok

Purification on gel :

-

Purification on gel :

-

Purification on gel :

-

digestion:

ClyA Cterm S/P

RFP Nterm X/P

PBAD vector : ? E/S


digestion:

ClyA Cter-RFP Nter vector PSB1A3 E/X

digestion:

-

digestion:

-

verification digestion:

ok

verification digestion:

ok

verification digestion:

-

verification digestion:

-


ligation:

ClyA Cter-RFP Nter vector PSB1A3

ligation:

PBAD E/S ClyA Cter-RFP Nter PSB1A3 E/X (x2) PBAD S/P ClyA Cter-RFP Nter PSB? X/P (x2)

ligation:

-

ligation:

-

PCR colo :

ok

PCR colo :

ok

PCR colo :

-

PCR colo :

-


miniprep:

clone :

miniprep:

clone :

miniprep:

-

miniprep:

-


sequencing :

ok

sequencing :

ok

sequencing :

-

sequencing :

-

stock glycerol:

S? S?

stock glycerol

S?

stock glycerol

-

stock glycerol

-



Export system

Time required : A week !



Experiments ran :

row 1 row 2 row 3
PCR :

TatABCE matrix :

PCR : PCR :


Verification on gel :

ok

Verification on gel :

-

Verification on gel :

-

Purification on gel :

ok

Purification on gel :

-

Purification on gel :

-


digestion:

TatABCE

digestion: digestion:
verification digestion:

ok

verification digestion:

-

verification digestion:

-


ligation:

TatABCE

ligation: ligation:


PCR colo :

ok

PCR colo :

-

PCR colo :

-

miniprep:

STOPPED

miniprep:

-

miniprep:

-

sequencing :

-

sequencing :

-

sequencing :

-

Retrieved from "http://2009.igem.org/Team:Paris/Addressing_testing"