Team:Paris/Addressing testing
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iGEM > Paris > WetLab > Addressing
Adressing system
global constructions :
Time required : A lOT !!!!!
Experiments ran :
row 1 | row 2 | row 3 |
PCR :
ClyA Cterm matrix : ? Oligo : TM : ClyA Nterm matrix : ? Oligo : TM : mRFP Cterm matrix : ? Oligo : TM : mRFP Nterm plasmid : ? Oligo : TM :
| PCR :
- | PCR :
- |
Verification on gel :
ok | Verification on gel :
- | Verification on gel :
- |
Purification on gel :
ok | Purification on gel :
- | Purification on gel :
-
|
digestion:
ClyA Cterm S/P RFP Nterm X/P PBAD vector : ? E/S
| digestion:
ClyA Cter-RFP Nter vector PSB1A3 E/X | digestion:
- |
verification digestion:
ok | verification digestion:
ok | verification digestion:
-
|
ligation:
ClyA Cter-RFP Nter vector PSB1A3 | ligation:
PBAD vector : ? E/S ClyA Cter-RFP Nter vector PSB1A3E/X (x2) PBAD vector : ? S/P ClyA Cter-RFP Nter X/P (x2) | ligation:
|
PCR colo :
ok | PCR colo :
ok | PCR colo :
-
|
miniprep:
clone : | miniprep:
clone : | miniprep:
-
|
sequencing :
ok | sequencing :
ok | sequencing :
- |
stock glycerol:
ok | stock glycerol
ok | stock glycerol
-
|
Pbad cut in S/P and ClyA Cter-RFP Nter in X/P
Pbad cut in E/S and ClyA Cter-RFP Nter in E/X
Export system
Time required : A lOT !!!!!
Experiments ran :
row 1 | row 2 | row 3 |
PCR :
| PCR : | PCR :
|
Verification on gel :
ok | Verification on gel :
ok | Verification on gel :
ok |
Purification on gel :
ok | Purification on gel :
ok | Purification on gel :
ok
|
digestion:
| digestion: | digestion: |
verification digestion:
ok | verification digestion:
ok | verification digestion:
ok
|
ligation:
| ligation: | ligation:
|
PCR colo :
ok | PCR colo :
ok | PCR colo :
ok
|
miniprep:
clone : | miniprep:
clone : | miniprep:
clone :
|
sequencing :
ok | sequencing :
ok | sequencing :
ok |