Team:Paris/Addressing testing
From 2009.igem.org
iGEM > Paris > WetLab > Addressing
Adressing system
global constructions :
Time required : A lOT !!!!!
Experiments ran :
row 1 | row 2 | row 3 |
PCR | PCR | PCR
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[] | 2003 | Miller SI & Guina T.
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[] | 2005 | Kuehn MJ & Kesty NC. |
[] | 2006 | McBroom AJ & Kuehn MJ. |
[] | 2007 | McBroom AJ & Kuehn MJ.
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[] | 2009 | Deatherage BL & Cookson BT.
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[] | 2009 | Purnick PE & Weiss R.
From plate: mRFP
ok Purification on gel ok digestion: ClyA Cterm S/P RFP Nterm X/P PBAD E/S
ClyA Cter-RFP Nter vector PSB1A3 PCR colo : miniprep: verifivation of the miniprep by digestion sequencing [[ClyA]] -ClyA Nterm= biobrick+sequencage+stock glycerol Done -ClyA Cterm= biobrick+sequencage+stock glycerol Done [[-RFP-]] -RFP Nterm= biobrick+sequencage+stock glycerol Done -RFP Cterm= biobrick+sequencage+stock glycerol Done [[-ClyA Cter-RFP Nter-]] -ClyA Cter-RFP Nter= biobrick+stock glycerol Done [[-RFP Cter-ClyA Nter-]] -miss ligation between 2 parts (j'ai fait plusieurs ligation le 7ou8/09, mais pas marché le 9/09 apres culture overnight, voir si les autres ont continué)
-failed many time with: Pbad cut in S/P and ClyA Cter-RFP Nter in X/P Pbad cut in E/S and ClyA Cter-RFP Nter in E/X
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