Team:Paris/Production design

From 2009.igem.org

(Difference between revisions)
(Parts)
(Parts)
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For this part we are going to use the plasmid backbone [http://partsregistry.org/Part:pSB2K3 pSB2K3].
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The function "increased production of OMV" is borne by the plasmid backbone [http://partsregistry.org/Part:pSB2K3 pSB2K3].
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The promoter pBAd used was found in the parts registry [http://partsregistry.org/Part:BBa_K113009 BBa_K11309].
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Expression of the constructs is controled by the Pbad promoter found in the parts registry [http://partsregistry.org/Part:BBa_K113009 BBa_K11309].
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We used the Ptet repressor [http://partsregistry.org/Part:BBa_R0040 BBa_ROO40] as wel as the coding sequence for the TetR protein [http://partsregistry.org/Part:BBa_C0040 BBa_COO40].
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We used the Ptet repressor [http://partsregistry.org/Part:BBa_R0040 BBa_ROO40] as well as the coding sequence for the TetR protein [http://partsregistry.org/Part:BBa_C0040 BBa_COO40].
The double terminator [http://partsregistry.org/Part:BBa_B0014 BBa_B0014] was found in the parts registry as well.
The double terminator [http://partsregistry.org/Part:BBa_B0014 BBa_B0014] was found in the parts registry as well.
The RBS were designed by Guillaume Cambray and integrated directly inside the primers.
The RBS were designed by Guillaume Cambray and integrated directly inside the primers.

Revision as of 11:58, 8 September 2009

iGEM > Paris > Production > Parts Design

Parts

ne serait-il pas bien de faire simplement un référencement des parts utilisées/créées dans cette partie et de renvoyer liens vers Freezer/NoteBook/Testing ?


The function "increased production of OMV" is borne by the plasmid backbone pSB2K3.


Expression of the constructs is controled by the Pbad promoter found in the parts registry BBa_K11309. We used the Ptet repressor BBa_ROO40 as well as the coding sequence for the TetR protein BBa_COO40. The double terminator BBa_B0014 was found in the parts registry as well.


The RBS were designed by Guillaume Cambray and integrated directly inside the primers.