Team:Paris/Production design

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iGEM > Paris > Production > Parts Design

Parts

The function "increased production of OMV" is borne by the plasmid backbone pSB2K3.


Expression of the constructs is controled by the Pbad promoter found in the parts registry BBa_K11309. We used the Ptet repressor BBa_ROO40 as well as the coding sequence for the TetR protein BBa_COO40. The double terminator BBa_B0014 was found in the parts registry as well.


The RBS were designed by Guillaume Cambray and integrated directly inside the primers.