Team:Paris/Protocols

Revision as of 15:32, 4 September 2009 by Rbodinier (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

Protocols Freezer NoteBook Bibliography Parts WetLab DryLab

Team Collaborations Contacts Acknowledgements Links

Contents 1 Protocols 2 Summary 2.1 1. Microscopy 2.2 2. Adapted Protocols 2.3 3. Culture protocols 2.4 4. Molecular biology

Protocols

Here you will find the collection of protocole we use, or just collect. And because we are well-known chauvinist it is just a mix with previous Paris iGEM team .

Main - Microscope Protocol - Adapted Protocols - Culture protocols - Molecular Biology Main

Summary

1. Microscopy

• Protocols suggested
• Protocol to membrane dying, DID method (pdf)

2. Adapted Protocols

• DNA extraction
• Mini prep
• Gel/PCR Purification

3. Culture protocols

• Over Night
• Bacterial transformation
• Competent bacteria
  • CaCl₂ (iGEM Paris2007)
  • CaCl₂ (2nd Protocol)
  • RbCl
• Glycerol stock
• Ferric citrate growth medium

4. Molecular biology

• PCR
• PCR colony
• Gels
• Digestion
• Ligation