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Team:Paris/Transduction modeling
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Our reception system uses the fec operon in order to transmit the signal received on the outer memebrane via the vesicules into the cytoplasm to induce a response from the reception cells. | Our reception system uses the fec operon in order to transmit the signal received on the outer memebrane via the vesicules into the cytoplasm to induce a response from the reception cells. | ||
| - | Once the vesicule has fusioned with the cell, the FecA molecule present on the previous vesicule wall activates the FecR molecule on the inner membrane ; then, | + | Once the vesicule has fusioned with the cell, the FecA molecule present on the previous vesicule wall activates the FecR molecule on the inner membrane ; then, the FecI molecule is activated and the pFec promoter is ON and transcription can start. |
| - | The plasmid of this reception cell was designed to produce a positive reaction once the signal is received so that, even with a very faint signal (very very few FecA molecules inside the vesicules), the reception population will be activated. To this end, we placed and | + | The plasmid of this reception cell was designed to produce a positive reaction once the signal is received so that, even with a very faint signal (very very few FecA molecules inside the vesicules), the reception population will be activated. To this end, we have placed the of FecR and the FecI coding which, once and |
===The Fec System as used in our system=== | ===The Fec System as used in our system=== | ||
Revision as of 15:08, 19 September 2009
iGEM > Paris > Reception > Modeling
Contents |
Modeling
Overview
Our reception system uses the fec operon in order to transmit the signal received on the outer memebrane via the vesicules into the cytoplasm to induce a response from the reception cells. Once the vesicule has fusioned with the cell, the FecA molecule present on the previous vesicule wall activates the FecR molecule on the inner membrane ; then, the FecI molecule is activated and the pFec promoter is ON and transcription can start. The plasmid of this reception cell was designed to produce a positive reaction once the signal is received so that, even with a very faint signal (very very few FecA molecules inside the vesicules), the reception population will be activated. To this end, we have placed the of FecR and the FecI coding which, once and
The Fec System as used in our system
Chemical Equations
Kinetics Equations
Stochastic and Deterministic Simulations
Results and discussion
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