Team:Paris/Transduction modeling

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Modeling

• Introduction
• A. The Fec Operon in our system
• B. Deterministic and Stochastic Simulations
• C. Results and discussion

Introduction

Our reception system uses the fec operon in order to transmit the signal received on the outer memebrane via the vesicules into the cytoplasm to induce a response of the reception cells. Once the vesicule has fusioned with the cell, the FecA molecule present on the previous vesicule wall activates the FecR molecule on the inner membrane ; then, the FecI molecule is activated and the pFec promoter is ON and transcription can start.

The plasmid of this reception cell was designed to produce a positive reaction once the signal is received so that, even with a very faint signal (very very few FecA molecules inside the vesicules), the reception population will be activated. To this end, we have placed the of FecR and the FecI coding downstream th epfec promoter. This way, once the first FecR and FecI molecules are produced, they take part in the activation of the pfec promoter thus creating a positive retroaction.

Our modelling study focussed on the description and analysis of this trancriptional cascade in order to caracterize the ability of the system to receive a signal. To this end, we tried to describe the system with elementary chemical equations allowing both deterministic and stochastic simulation runs. To get more information on this study, please visit the following sections :

• The Fec Operon in our system to read more about the chemical equations chosen to describe the system and the kinetics equations associated
• Deterministic and Stochastic Simulations to read more about the different simulations.
• Results and discussion to learn more about the results of this study.