Team:Paris/Transduction overview

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Overview

• Introduction
• A. Fusion
  • A.1 jun/fos
  • A.2 G3P
  • A.3 Snares
• B. Transduction
  • B.1 ABC transporters
  • B.2 Two Component System
• C. Our strategy
• D. Construction

Introduction

This part of the project was focus on two points:

• The fusion between the OMVs and the targeted bacteria.

• The activation of the transcription of a genetic construction after fusionning the OMVs with the outer membrane of the receiving bacterium.

The sinequanone conditions : we tried to achieve this aim without sacrifying important proprieties of our message : specific, repeatable , multidirectional.

It seems that we have two possible ways : the ABC transporters or the two component systems .

ABC transporters and two component systems are natural transport systems (export or import) of nutriments or toxines, and even information, the mecanism is mostly unknown but the DNA-containing OMVs could be a useful mean of information transport. Here for information about ^ABC transporter and Two-component system

References

1. ^ D-ribose metabolism in Escherichia coli K-12: genetics, regulation, and transport. Lopilato JE & Beckwith JR. 1984 - [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=6327616 6327616]
2. ^ Signal transfer through three compartments: transcription initiation of the Escherichia coli ferric citrate transport system from the cell surface. Härle C & Braun V. 1995 - [http://www.ncbi.nlm.nih.gov/pubmed/7729419 7729419]
3. ^ A comparative analysis of ABC transporters in complete microbial genomes. Tomii K & Kanehisa M. 1998- [http://www.ncbi.nlm.nih.gov/pubmed/9799792 9799792]
4. ^ The CpxRA signal transduction system of Escherichia coli: growth-related autoactivation and control of unanticipated target operons.De Wulf P & Lin EC. 1999 - [http://www.ncbi.nlm.nih.gov/pubmed/10542180 10542180]
5. ^ Two-component signal transduction. Stock AM & Goudreau PN. 2000- [http://www.ncbi.nlm.nih.gov/pubmed/10966457 10966457]
6. ^ Vesicle-mediated transfer of virulence genes from Escherichia coli O157:H7 to other enteric bacteria. Yaron S & Matthews KR. 2000- [http://www.ncbi.nlm.nih.gov/pubmed/11010892 11010892]
7. ^ Periplasmic binding proteins: a versatile superfamily for protein engineering. Dwyer MA & Hellinga HW. 2004- [http://www.ncbi.nlm.nih.gov/pubmed/11010892 11010892]
8. ^ Gene regulation by transmembrane signaling. Braun V & Sauter A. 2006- [http://www.ncbi.nlm.nih.gov/pubmed/16718597 16718597]
9. ^ Signal transduction: networks and integrated circuits in bacterial cognition. Baker MD & Stock JB 2007- [http://www.ncbi.nlm.nih.gov/pubmed/18054766 18054766]
10. ^ Systems biology of bacterial chemotaxis. Baker MD & Stock JB. 2007- [http://www.ncbi.nlm.nih.gov/pubmed/16529985 16529985]
11. ^ Control of the transcription of a short gene encoding a cyclic peptide in Streptococcus thermophilus: a new quorum-sensing system? Ibrahim M & Monnet V. 2007- [http://www.ncbi.nlm.nih.gov/pubmed/17921293 17921293]
12. ^ SRP and Sec pathway leader peptides for antibody phage display and antibody fragment production in E. coli. Thie H & Hust M. 2008- [http://www.ncbi.nlm.nih.gov/pubmed/18504019 18504019]
13. ^ Signal transduction and adaptive regulation through bacterial two-component systems: the Escherichia coli AtoSC paradigm. Kyriakidis DA & Tiligada E. 2009- [http://www.ncbi.nlm.nih.gov/pubmed/19198978 19198978]
14. ^ comparative analysis of ABC transporter. Tomii & Kanehisa 2009- [http://genome.cshlp.org/content/8/10/1048.full.html#ref-list-1 pdf-link]