Team:Paris/Transduction overview
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Introduction
This part of the project was focus on two points:
- The fusion between the OMVs and the targeted bacteria.
- The activation of the transcription of a genetic construction after fusionning the OMVs with the outer membrane of the receiving bacterium.
The sinequanone conditions : we tried to achieve this aim without sacrifying important proprieties of our message : specific, repeatable , multidirectional.
It seems that we have two possible ways : the ABC transporters or the two component systems .
ABC transporters and two component systems are natural transport system (export or import) of nutriments or toxines. There is another possibility but the mecanism is mostly unknown : the DNA-containing OMVs which could be a useful mean of information transport.
References
- ^ D-ribose metabolism in Escherichia coli K-12: genetics, regulation, and transport. Lopilato JE & Beckwith JR. 1984 - 6327616
- ^ Signal transfer through three compartments: transcription initiation of the Escherichia coli ferric citrate transport system from the cell surface. Härle C & Braun V. 1995 - 7729419
- ^ A comparative analysis of ABC transporters in complete microbial genomes. Tomii K & Kanehisa M. 1998- 9799792
- ^ The CpxRA signal transduction system of Escherichia coli: growth-related autoactivation and control of unanticipated target operons.De Wulf P & Lin EC. 1999 - 10542180
- ^ Two-component signal transduction. Stock AM & Goudreau PN. 2000- 10966457
- ^ Vesicle-mediated transfer of virulence genes from Escherichia coli O157:H7 to other enteric bacteria. Yaron S & Matthews KR. 2000- 11010892
- ^ Periplasmic binding proteins: a versatile superfamily for protein engineering. Dwyer MA & Hellinga HW. 2004- 11010892
- ^ Gene regulation by transmembrane signaling. Braun V & Sauter A. 2006- 16718597
- ^ Signal transduction: networks and integrated circuits in bacterial cognition. Baker MD & Stock JB 2007- 18054766
- ^ Systems biology of bacterial chemotaxis. Baker MD & Stock JB. 2007- 16529985
- ^ Control of the transcription of a short gene encoding a cyclic peptide in Streptococcus thermophilus: a new quorum-sensing system? Ibrahim M & Monnet V. 2007- 17921293
- ^ SRP and Sec pathway leader peptides for antibody phage display and antibody fragment production in E. coli. Thie H & Hust M. 2008- 18504019
- ^ Signal transduction and adaptive regulation through bacterial two-component systems: the Escherichia coli AtoSC paradigm. Kyriakidis DA & Tiligada E. 2009- 19198978
- ^ comparative analysis of ABC transporter. Tomii & Kanehisa 2009- pdf-link