Team:Paris/Transduction overview construction

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<span/ id="bottom">[https://2009.igem.org/ iGEM ] > [[Team:Paris#top | Paris]] > [[Team:Paris/Transduction_overview#top | Reception]] > [[Team:Paris/Transduction_overview_construction#bottom | Construction]]
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<span/ id="bottom">[https://2009.igem.org/ iGEM ] > [[Team:Paris#top | Paris]] > [[Team:Paris/Transduction_overview#top | Receiving the message]] > [[Team:Paris/Transduction_overview_construction#bottom | Construction]]
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== Overview  ==
 
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* [[Team:Paris/Transduction_overview#Overview#bottom | Introduction]]
 
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* [[Team:Paris/Transduction_overview_fusion#bottom |A. Fusion ]]
 
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** [[Team:Paris/Transduction_overview_fusion#A.1 Jun/Fos|A.1 jun/fos]]
 
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** [[Team:Paris/Transduction_overview_fusion#A.2 G3P|A.2 G3P]]
 
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** [[Team:Paris/Transduction_overview_fusion#A.3 Snares |A.3 Snares]]
 
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* [[Team:Paris/Transduction_overview_transduction#bottom |B. Transduction]]
 
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** [[Team:Paris/Transduction_overview_transduction#B.1 ABC transporters|B.1 ABC transporters]]
 
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** [[Team:Paris/Transduction_overview_transduction#B.2 Two-component system|B.2  Two Component System]]
 
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* [[Team:Paris/Transduction_overview_strategy#bottom |C. Our strategy]]
 
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* [[Team:Paris/Transduction_overview_construction#bottom |D. Construction]]
 
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==Construction==
<html>
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    background: url(https://static.igem.org/mediawiki/2009/1/1b/Left_menu_pari.png);
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===D. Construction===
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#middle-side {
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<b class="correction"> blabla grossiste sur la Transduction et de ses possibles façon d'exister. Montrer la présence de ABCT, TCS ou autre truc qui existe, et dire qu'on va détailler les 2 premiers</b>
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<a class="menu_sub"href="https://2009.igem.org/Team:Paris/Transduction_overview#bottom"> Main </a>|
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<a class="menu_sub"href="https://2009.igem.org/Team:Paris/Transduction_overview_fusion#bottom"> Fusion</a>|
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<a class="menu_sub"href="https://2009.igem.org/Team:Paris/Transduction_overview_strategy#bottom"> Our strategy</a>|
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<a class="menu_sub_active"href="https://2009.igem.org/Team:Paris/Transduction_overview_construction#bottom"> Construction</a>
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====D.1 Proof of the activity of pfec====
 
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After all the process to obtain pfec as a biobrick, we have to clone it into BBa j61002 which allows to test promotor efficiency.
 
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After the transformation of this plasmid into a FecA + strains and on a medium were the ferric citrate is the only iron source, the RFP should be expressed and visible.
 
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Construction required for the fusion of the vesicles that contain the message:
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G3P , OmpAL , Jun, Fos , and AIDA protein.
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====reference====
 
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<b class="correction"> elles sont ou exactement ? </b>
 
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#1995- Cosima Harle & Volkmar Braun - Signal transfer through three compartments transcription initiation of the Escherichia coiferric  citrate transport system from the cell surface
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#2000 - Stock & Goudreau – Two-component signal  transduction
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[[Image:Paris_construc_7_aida.jpg |700px|center|plasmid = ]]
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#2001 - Mishima & Murata - Super-channel in bacteria  function and structure of the macromolecule import system mediated by a pit-dependent ABC transporter
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#2009 - Akyriakidis & Tiligada – signal transduction TCS the AtoSC paradigm
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#2009 - Tomii & Kanehisa – comparative analysis of ABC transporter
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[[Image:Paris_construc_8_junfos.jpg |700px|center|plasmid = ]]
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<a href="https://2009.igem.org/Team:Paris/Transduction_overview_construction#bottom"><img style="width:40px; height:40px;" src="https://static.igem.org/mediawiki/2009/1/10/Paris_Up.png"/></a>
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</html>

Latest revision as of 14:11, 21 October 2009

iGEM > Paris > Receiving the message > Construction


Construction


Construction required for the fusion of the vesicles that contain the message:

G3P , OmpAL , Jun, Fos , and AIDA protein.



plasmid =




plasmid =



Retrieved from "http://2009.igem.org/Team:Paris/Transduction_overview_construction"