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Team:Paris/Transduction overview construction
From 2009.igem.org
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| + | After all the process to obtain pfec as a biobrick, we have to clone it into BBa j61002 which allows to test promotor efficiency. | ||
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Revision as of 10:24, 2 October 2009
iGEM > Paris > Reception > Construction
D. Construction
blabla grossiste sur la Transduction et de ses possibles façon d'exister. Montrer la présence de ABCT, TCS ou autre truc qui existe, et dire qu'on va détailler les 2 premiers
D.1 Proof of the activity of pfec
After all the process to obtain pfec as a biobrick, we have to clone it into BBa j61002 which allows to test promotor efficiency. After the transformation of this plasmid into a FecA + strains and on a medium were the ferric citrate is the only iron source, the RFP should be expressed and visible.
reference
elles sont ou exactement ?
- 1995- Cosima Harle & Volkmar Braun - Signal transfer through three compartments transcription initiation of the Escherichia coiferric citrate transport system from the cell surface
- 2000 - Stock & Goudreau – Two-component signal transduction
- 2001 - Mishima & Murata - Super-channel in bacteria function and structure of the macromolecule import system mediated by a pit-dependent ABC transporter
- 2009 - Akyriakidis & Tiligada – signal transduction TCS the AtoSC paradigm
- 2009 - Tomii & Kanehisa – comparative analysis of ABC transporter
