Team:Paris/Transduction overview strategy

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<span/ id="bottom">[https://2009.igem.org/ iGEM ] > [[Team:Paris#top | Paris]] > [[Team:Paris/Transduction_overview#top | Reception]] > [[Team:Paris/Transduction_overview_strategy#bottom | Our strategy]]
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<span/ id="bottom">[https://2009.igem.org/ iGEM ] > [[Team:Paris#top | Paris]] > [[Team:Paris/Transduction_overview#top | Receiving the message]] > [[Team:Paris/Transduction_overview_strategy#bottom | Our strategy]]
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== Overview  ==
 
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* [[Team:Paris/Transduction_overview#Overview#bottom | Introduction]]
 
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* [[Team:Paris/Transduction_overview_fusion#bottom |A. Fusion ]]
 
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** [[Team:Paris/Transduction_overview_fusion#A.1 Jun/Fos|A.1 jun/fos]]
 
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** [[Team:Paris/Transduction_overview_fusion#A.2 G3P|A.2 G3P]]
 
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** [[Team:Paris/Transduction_overview_fusion#A.3 Snares |A.3 Snares]]
 
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* [[Team:Paris/Transduction_overview_strategy#bottom |B. Our strategy]]
 
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* [[Team:Paris/Transduction_overview_construction#bottom |C. Construction]]
 
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===C. Our Strategy===
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==Membran Fusion : Our Strategy==
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<a class="menu_sub"href="https://2009.igem.org/Team:Paris/Transduction_overview#bottom"> Main </a>|
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<a class="menu_sub"href="https://2009.igem.org/Team:Paris/Transduction_overview_fusion#bottom"> Fusion</a>|
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<a class="menu_sub_active"href="https://2009.igem.org/Team:Paris/Transduction_overview_strategy#bottom"> Our strategy</a>|
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====B.1 ? ====
 
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euh
 
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====C.2 The trick TCS====
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===Jun/Fos strategy===
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Our strategy is to fuse the mutated Jun (the one unable to form homodimer) to AIDA, the autotransporter (for the donnor cell). By this way, Jun will be export to the outer membrane and should be on vesicles.
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The second step is to make a fusion between Fos and AIDA (for the receiver bacteria). In this direction Fos will be trasport to the outer membrane .
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===g3p strategy===
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g3p is naturally exposed at the surface of the filamentous bacteriophage. The M13 phage has a high affinity for E.coli, and if we could place its g3p on the surface of the vesicles it could activate the fusion with the Outer membrane of the targeted bacteria.
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Outer membrane protein A (OmpA) is a major structural protein of the outer membrane of Escherichia coli.
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To conclude, our strategy is to fuse OmpA-Linker to g3p to export the g3p protein to the outer membrane in order to find them on the vesicles surface.
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We could ,with the help of Alfonso Jaramillo, design a synthetic PBP which could detect the substrate we choosed and activate a specific HK.
 
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The previous work done by Valencia in 2006 was to design vanilin-sensitive PBP and a network for a graduated response whereas we just need a proteic sensitive PBP and a binary type of response.
 
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Even if the idea of creating a synthethic protein was very attractive, it would have been difficult to have concrete result in just 3 months.
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Latest revision as of 22:41, 21 October 2009

iGEM > Paris > Receiving the message > Our strategy


Membran Fusion : Our Strategy


Jun/Fos strategy

Our strategy is to fuse the mutated Jun (the one unable to form homodimer) to AIDA, the autotransporter (for the donnor cell). By this way, Jun will be export to the outer membrane and should be on vesicles. The second step is to make a fusion between Fos and AIDA (for the receiver bacteria). In this direction Fos will be trasport to the outer membrane .

g3p strategy

g3p is naturally exposed at the surface of the filamentous bacteriophage. The M13 phage has a high affinity for E.coli, and if we could place its g3p on the surface of the vesicles it could activate the fusion with the Outer membrane of the targeted bacteria.


Outer membrane protein A (OmpA) is a major structural protein of the outer membrane of Escherichia coli.


To conclude, our strategy is to fuse OmpA-Linker to g3p to export the g3p protein to the outer membrane in order to find them on the vesicles surface.


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