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Team:Paris/Transduction overview strategy
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* [[Team:Paris/Transduction_overview_strategy#bottom |C. Our strategy]] | * [[Team:Paris/Transduction_overview_strategy#bottom |C. Our strategy]] | ||
* [[Team:Paris/Transduction_overview_construction#bottom |D. Construction]] | * [[Team:Paris/Transduction_overview_construction#bottom |D. Construction]] | ||
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| + | ====C.x Fec==== | ||
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| + | Few ABC transporter such as FecABCD (iron transporter) are able to induce a response regardless of the tranlocation, due to the activity of FecA, some mutant can also have a constitutive expression of FecABCD . We could use FecA- mutant receiver | ||
| + | and FecA+ mutant donor to transfert working FecA to the receiver. In this case the receiver will express the FecABCD operon, and so we could place under the control of the Fec ABCD promoter the gene sequence encoding for the response. | ||
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| + | [[Image:Transduction_overview_fec.png|500px|center|thumb| fec operon induction]] | ||
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| + | '''problems :''' the message is unidirectionnal and unrepeatable. It would just be a proof of principle that a vesicle-mediated controlled communication is possible. | ||
Revision as of 20:06, 2 September 2009
iGEM > Paris > Reception > Our strategy
Overview
C.x Fec
Few ABC transporter such as FecABCD (iron transporter) are able to induce a response regardless of the tranlocation, due to the activity of FecA, some mutant can also have a constitutive expression of FecABCD . We could use FecA- mutant receiver and FecA+ mutant donor to transfert working FecA to the receiver. In this case the receiver will express the FecABCD operon, and so we could place under the control of the Fec ABCD promoter the gene sequence encoding for the response.
